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一种用于检测粪便样本中溶组织内阿米巴的抗原捕获酶免疫测定法的评估。

Evaluation of an antigen-capture enzyme immunoassay for detection of Entamoeba histolytica in stool samples.

作者信息

Jelinek T, Peyerl G, Löscher T, Nothdurft H D

机构信息

Department of Infectious Diseases and Tropical Medicine, University of Munich, Germany.

出版信息

Eur J Clin Microbiol Infect Dis. 1996 Sep;15(9):752-5. doi: 10.1007/BF01691966.

DOI:10.1007/BF01691966
PMID:8922579
Abstract

In order to identify the prevalence of Entamoeba histolytica in tourists with diarrhoea returning from countries of the developing world, sensitivity and specificity of a commercially available enzyme immunoassay (EIA) kit for the detection of Entamoeba histolytica coproantigen in stool were evaluated. Five hundred seventy-seven specimens from 469 patients were examined by microscopy and EIA. Sixty-two specimens from 49 patients were considered positive for Entamoeba histolytica. Compared with microscopic examination of stool samples, the EIA was found to be slightly more sensitive (90.3% vs. 87.1%) and was 97.7% specific for Entamoeba histolytica.

摘要

为了确定从发展中国家归来的腹泻游客中溶组织内阿米巴的流行情况,对一种市售酶免疫分析(EIA)试剂盒检测粪便中溶组织内阿米巴粪抗原的敏感性和特异性进行了评估。对469例患者的577份标本进行了显微镜检查和EIA检测。49例患者的62份标本被认为溶组织内阿米巴呈阳性。与粪便样本的显微镜检查相比,发现EIA的敏感性略高(90.3%对87.1%),对溶组织内阿米巴的特异性为97.7%。

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Short report: detection of Entamoeba histolytica and E. dispar directly in stool.简短报告:直接在粪便中检测溶组织内阿米巴和迪斯帕内阿米巴。
Am J Trop Med Hyg. 1994 May;50(5):595-6. doi: 10.4269/ajtmh.1994.50.595.
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Use of a monoclonal antibody in an enzyme immunoassay for the detection of Entamoeba histolytica in fecal specimens.一种单克隆抗体在酶免疫测定法中用于检测粪便标本中的溶组织内阿米巴。
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Development of simple and stable ELISA for detection of copro antigen in intestinal amoebiasis.用于检测肠道阿米巴病粪便抗原的简单稳定酶联免疫吸附测定法的开发。
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