原发性中枢神经系统大B细胞淋巴瘤中人疱疹病毒8感染的免疫组织化学评估
Immunohistochemical assessment of human herpesvirus 8 infection in primary central nervous system large B cell lymphomas.
作者信息
Gomez-Brouchet A, Delisle M B, Vital A, Brousset P
机构信息
Department of Pathology, CHU Rangueil, 31403 Toulouse, France.
出版信息
J Clin Pathol. 2001 Aug;54(8):617-8. doi: 10.1136/jcp.54.8.617.
BACKGROUND/AIMS: Primary central nervous system large B cell lymphomas (PCNSLs) are frequently associated with Epstein-Barr virus (EBV) in patients with AIDS and less frequently in those without AIDS. Human herpesvirus 8 (HHV-8) has been detected in these tumours by the polymerase chain reaction (PCR) at low copy number, suggesting its presence in a cell compartment other than the malignant one. The aim of this study was to use immunohistochemistry to assess HHV-8 infection in a series of PCNSLs from patients with and without AIDS.
METHODS
The antibody LN53, which reacts with the latent nuclear antigen 1 (LNA1) of HHV-8, was used on tissue sections from 35 patients (17 with and 18 without AIDS) with PCNSL. In addition, DNA was available for PCR (open reading frame 26 (ORF 26), ORF 72, ORF 75) in three patients (two without AIDS, one with AIDS).
RESULTS
None of the 35 cases contained either DNA sequences or LNA1 positive cells.
CONCLUSIONS
These results confirm the lack of HHV-8 infection in tumour cells of PCNSL. In addition, HHV-8 infected bystander cells do not express LNA1 latent protein in this setting.
背景/目的：原发性中枢神经系统大B细胞淋巴瘤（PCNSL）在艾滋病患者中常与爱泼斯坦-巴尔病毒（EBV）相关，在非艾滋病患者中相关性较低。通过聚合酶链反应（PCR）已在这些肿瘤中检测到低拷贝数的人类疱疹病毒8（HHV-8），提示其存在于除恶性细胞外的细胞区室中。本研究的目的是使用免疫组织化学评估一系列艾滋病患者和非艾滋病患者的PCNSL中HHV-8感染情况。
方法
使用与HHV-8的潜伏核抗原1（LNA1）反应的抗体LN53，对35例PCNSL患者（17例艾滋病患者和18例非艾滋病患者）的组织切片进行检测。此外，3例患者（2例非艾滋病患者，1例艾滋病患者）可获得用于PCR（开放阅读框26（ORF 26）、ORF 72、ORF 75）的DNA。
结果
35例病例中均未检测到DNA序列或LNA1阳性细胞。
结论
这些结果证实PCNSL肿瘤细胞中不存在HHV-8感染。此外，在这种情况下，HHV-8感染的旁观者细胞不表达LNA1潜伏蛋白。
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