Gaidano G, Capello D, Pastore C, Antinori A, Gloghini A, Carbone A, Larocca L M, Saglio G
Dipartimento di Scienze Biomediche e Oncologia Umana, Università di Torino, Turin, Italy.
J Infect Dis. 1997 May;175(5):1193-7. doi: 10.1086/593456.
Human herpesvirus type 8 (HHV-8) has been proposed as a pathogenetic factor for immunosuppression-associated primary central nervous system lymphoma (PCNSL). To verify this hypothesis, HHV-8 infection was investigated in 31 persons with PCNSL (16 AIDS-related, 15 AIDS-unrelated) and in 30 persons with systemic B cell non-Hodgkin's lymphomas (B-NHL; 15 AIDS-related, 15 AIDS-unrelated). All subjects with PCNSL scored negative by single-step polymerase chain reaction (PCR), suggesting a tumor virus load of <100 viral copies/200,000 human haploid genome equivalents (HHGE). By applying Poisson assumptions to nested PCR, 16 of 31 persons with PCNSL were devoid of HHV-8 sequences: 1 subject with AIDS and PCNSL had 1-100 viral copies/200,000 HHGE, and 14 with PCNSL had <1 viral copy/200,000 HHGE. Similarly, 10 of 30 persons with systemic B-NHL were devoid of HHV-8 sequences; 20 had <1 viral copy/200,000 HHGE. The extremely low levels of infection rule out a role of HHV-8 in PCNSL pathogenesis and are consistent with HHV-8 infection of bystander cells contaminating the tumor clone.
人疱疹病毒8型（HHV-8）被认为是免疫抑制相关原发性中枢神经系统淋巴瘤（PCNSL）的致病因素。为验证这一假说，对31例PCNSL患者（16例与艾滋病相关，15例与艾滋病无关）和30例系统性B细胞非霍奇金淋巴瘤（B-NHL；15例与艾滋病相关，15例与艾滋病无关）患者进行了HHV-8感染调查。所有PCNSL患者通过单步聚合酶链反应（PCR）检测均为阴性，提示肿瘤病毒载量<100个病毒拷贝/200,000个人单倍体基因组当量（HHGE）。通过将泊松假设应用于巢式PCR，31例PCNSL患者中有16例没有HHV-8序列：1例艾滋病合并PCNSL患者有1 - 100个病毒拷贝/200,000 HHGE，14例PCNSL患者的病毒拷贝数<1个/200,000 HHGE。同样，30例系统性B-NHL患者中有10例没有HHV-8序列；20例的病毒拷贝数<1个/200,000 HHGE。极低的感染水平排除了HHV-8在PCNSL发病机制中的作用，与旁观者细胞的HHV-8感染污染肿瘤克隆一致。
