Asperio R M, Nicolato E, Marzola P, Farace P, Lunati E, Sbarbati A, Osculati F
Department of Morphological-Biomedical Sciences, Institute of Anatomy and Histology, University of Verona, Medical Faculty, Strada Le Grazie 8, 37134 Verona, Italy.
Radiology. 2001 Aug;220(2):413-9. doi: 10.1148/radiology.220.2.r01au03413.
To evaluate whether the vascular system resulting from an arterial lesion shows differences in permeability to a tracer with respect to the normal vascular system and whether eventual differences are maintained for long periods.
Permanent ischemia was induced in rats with femoral arterial removal, and magnetic resonance (MR) imaging was performed after 1, 7, 14, and 90 days. Gadopentetate dimeglumine was injected, and the kinetics of its penetration in the leg were studied. Phosphorus 31 spectroscopy was performed to determine the bioenergetic characteristics of the gastrocnemius muscle at rest and stimulation. Ischemic muscles were then processed for electron microscopy.
After ischemia induction, a hyperintense area that progressively decreased was present on T2-weighted images. Gadopentetate dimeglumine improved the signal intensity of the area. Three months after arterial occlusion, the contrast-enhanced images still showed microvessels highly permeable to the tracers. Spectroscopic data revealed that 3 months after arterial removal, the bioenergetic reserve of the gastrocnemius muscle was reduced, suggesting that the contrast-enhanced MR imaging-visible area is functionally relevant. Ultrastructural examination revealed persistent muscle damage and signs of chronic microangiopathy.
After ischemia induction, the restitutio ad integrum is not complete, and delayed muscle injuries can result from arterial insufficiency.
评估因动脉病变导致的血管系统在对示踪剂的通透性方面是否与正常血管系统存在差异，以及最终的差异是否会长期持续存在。
通过切除大鼠股动脉诱导永久性缺血，并在1、7、14和90天后进行磁共振（MR）成像。注射钆喷酸葡胺，研究其在腿部的渗透动力学。进行磷31光谱分析以确定腓肠肌在静息和刺激状态下的生物能量特征。然后对缺血肌肉进行电子显微镜检查。
缺血诱导后，T2加权图像上出现一个逐渐减小的高信号区域。钆喷酸葡胺改善了该区域的信号强度。动脉闭塞三个月后，对比增强图像仍显示微血管对示踪剂具有高通透性。光谱数据显示，动脉切除三个月后，腓肠肌的生物能量储备减少，这表明对比增强MR成像可见区域具有功能相关性。超微结构检查显示持续的肌肉损伤和慢性微血管病迹象。
缺血诱导后，恢复并不完全，动脉供血不足可导致延迟性肌肉损伤。
