Bas S, Muzzin P, Fulpius T, Buchs N, Vischer T L
Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland.
Rheumatology (Oxford). 2001 Jul;40(7):801-5. doi: 10.1093/rheumatology/40.7.801.
To investigate whether B-cell stimulation occurs in joints of Chlamydia trachomatis reactive arthritis patients by comparing the immunoglobulin G (IgG) anti-C. trachomatis antibody responses in serum and synovial fluid (SF).
The number and spectrum of C. trachomatis antigens recognized by paired serum and SF samples from 16 patients with C. trachomatis reactive arthritis and 20 patients with other inflammatory arthropathies independent of this bacteria, were studied by immunoblotting. The responses to five different Chlamydia antigens were also determined in enzyme-linked immunosorbent assays.
In C. trachomatis reactive arthritis patients, a higher number of C. trachomatis antigens was recognized by SF (17.6+/-5.1) than by serum (11.1+/-6.3) IgG and a higher intensity of SF IgG binding to the outer membrane protein 2 (OMP2) was observed.
These results suggest an intra-articular IgG production and a possible role of some Chlamydia antigens like OMP2 in the pathogenesis of C. trachomatis reactive arthritis.
通过比较沙眼衣原体反应性关节炎患者血清和滑液（SF）中抗沙眼衣原体免疫球蛋白G（IgG）抗体反应，研究沙眼衣原体反应性关节炎患者关节中是否发生B细胞刺激。
采用免疫印迹法研究16例沙眼衣原体反应性关节炎患者和20例与该细菌无关的其他炎性关节病患者的配对血清和SF样本所识别的沙眼衣原体抗原的数量和谱。还通过酶联免疫吸附试验测定了对五种不同衣原体抗原的反应。
在沙眼衣原体反应性关节炎患者中，SF识别的沙眼衣原体抗原数量（17.6±5.1）高于血清（11.1±6.3）IgG，并且观察到SF IgG与外膜蛋白2（OMP2）的结合强度更高。
这些结果表明关节内IgG产生以及某些衣原体抗原如OMP2在沙眼衣原体反应性关节炎发病机制中可能起的作用。
