[培养的子宫内膜异位症细胞中单核细胞趋化蛋白-1的测定]

[Determination of monocyte chemotactic protein-1 in cultured endometriotic cells].

作者信息

Gao Y, Luo L, He F

机构信息

Union Hospital, Tongji Medical University, Wuhan 430022.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 1999 Oct;34(10):612-4.

PMID:11477802

Abstract

OBJECTIVE

To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues.

METHODS

Expressions of MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis, immunohistochemical method [streptavidin biotin-peroxidase complex (SABC)] and enzyme linked immunosorbent assay (ELISA) methods in cultured endometriotic cells with median (controls) or with interleukin-1 beta(IL-1 beta) 2 ng/ml (IL-1 beta group), and tumor necrosis factor-alpha(TNF-alpha) 20 mg/ml(TNF-alpha group) respectively. The endometriotic tissues were sourced from 15 patients with endometriosis. Meanwhile, the MCP-1 protein content of cultured supernatent was also measured.

RESULTS

After exposured to IL-1 beta or TNF-alpha, the expression of MCP-1 mRNA and MEP-1 protein in the endometriotic cells were significantly higher than that in the control group (P < 0.01, P < 0.05 respectively); so was the MCP-1 protein content of supernatent.

CONCLUSIONS

IL-1 beta and TNF-alpha can up-regulate the expression of MCP-1 in endometriotic cells, which may be related to the development of endometriosis.

摘要

目的

探讨子宫内膜异位症组织产生的单核细胞趋化蛋白-1（MCP-1）的临床意义。

方法

采用斑点印迹分析、免疫组织化学方法[链霉亲和素生物素-过氧化物酶复合物（SABC）]和酶联免疫吸附测定（ELISA）法，分别检测培养的子宫内膜异位症细胞（中位数为对照组）、添加2 ng/ml白细胞介素-1β（IL-1β组）和20 mg/ml肿瘤坏死因子-α（TNF-α组）中MCP-1 mRNA和MCP-1蛋白的表达。子宫内膜异位症组织取自15例子宫内膜异位症患者。同时，检测培养上清液中MCP-1蛋白含量。