Gao Y, Luo L, He F
Department of Obstetrics and Gynecology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
J Tongji Med Univ. 1999;19(3):212-4. doi: 10.1007/BF02887737.
To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin-1 beta (IL-1 beta, 2 micrograms/L), tumor necrosis factor-alpha (TNF-alpha, 20 g/L). After exposure to IL-1 beta or TNF-alpha, the expression of MCP-1 mRNA in the endometriotic cells (8.635 +/- 0.826, 7.031 +/- 0.970, respectively) were significantly higher than that in the control group (4.482 +/- 0.435, P < 0.05); The expression of MCP-1 protein in IL-1 beta and TNF-alpha group was 4.52 +/- 0.09 micrograms/L, 2.87 +/- 0.27 micrograms/L, respectively, which were significantly higher than 1.74 +/- 0.16 micrograms/L in control (P < 0.01). The results suggested that IL-1 beta and TNF-alpha could up-regulate the expression of MCP-1 in endometriotic cells, which might be related to the development of endometriosis.
为研究子宫内膜异位症组织产生的单核细胞趋化蛋白-1（MCP-1）的临床意义，从15例子宫内膜异位症患者身上获取子宫内膜异位症组织。采用斑点杂交分析和酶联免疫吸附测定（ELISA）法，检测在有或无白细胞介素-1β（IL-1β，2微克/升）、肿瘤坏死因子-α（TNF-α，20微克/升）情况下培养的子宫内膜异位症细胞中的MCP-1 mRNA和MCP-1蛋白。暴露于IL-1β或TNF-α后，子宫内膜异位症细胞中MCP-1 mRNA的表达（分别为8.635±0.826、7.031±0.970）显著高于对照组（4.482±0.435，P<0.05）；IL-1β组和TNF-α组中MCP-1蛋白的表达分别为4.52±0.09微克/升、2.87±0.27微克/升，显著高于对照组的1.74±0.16微克/升（P<0.01）。结果表明，IL-1β和TNF-α可上调子宫内膜异位症细胞中MCP-1的表达，这可能与子宫内膜异位症的发生发展有关。
