Lin J D, Hsueh C, Chao T C, Weng H F
Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C.
Endocr Pathol. 2001 Spring;12(1):15-21. doi: 10.1385/ep:12:1:15.
The extent of human sodium iodide symporter (hNIS) expression in different kinds of human thyroid cancer tissues and cell lines remains controversial. In this study, polyclonal antibodies to hNIS were used to analyze the expression of symporter protein in benign and malignant human thyroid tissues. Formalin-fixed, paraffin wax-embedded tissue sections were used. Staining was performed using primary polyclonal antibody of rabbit anti-human hNIS diluted in PBS (1:500). Results showed that 2 of 3 normal tissue, 3 of 6 nodular hyperplasia, one follicular adenoma, 3 of 11 papillary thyroid carcinoma, 1 of 5 follicular carcinoma and none of 3 metastatic thyroid epithelial tissue specimens stained positively for hNIS. A higher percentage of positive staining for symporter protein was found in benign thyroid tissues including normal thyroid tissue, nodular hyperplasia, and adenoma (60%). In contrast, papillary and follicular thyroid carcinomas demonstrated lower symporter protein expression (20%). In conclusion, although the number of tissue samples examined in this study was small, hNIS staining found a higher ratio of symporter protein expression in normal and benign thyroid tissues compared with malignant tissues. Determination of the reason for discrepancies in the expression of hNIS in in vivo and in vitro studies will require further investigation.
人钠碘转运体（hNIS）在不同类型的人甲状腺癌组织和细胞系中的表达程度仍存在争议。在本研究中，使用针对hNIS的多克隆抗体分析人甲状腺良恶性组织中转运体蛋白的表达。采用福尔马林固定、石蜡包埋的组织切片。使用在PBS中稀释的兔抗人hNIS多克隆一抗（1:500）进行染色。结果显示，3份正常组织中有2份、6份结节性增生中有3份、1份滤泡性腺瘤、11份乳头状甲状腺癌中有3份、5份滤泡癌中有1份以及3份转移性甲状腺上皮组织标本中均未检测到hNIS阳性染色。在包括正常甲状腺组织、结节性增生和腺瘤在内的良性甲状腺组织中，转运体蛋白阳性染色的比例更高（60%）。相比之下，乳头状和滤泡状甲状腺癌的转运体蛋白表达较低（20%）。总之，尽管本研究中检测的组织样本数量较少，但hNIS染色发现正常和良性甲状腺组织中转运体蛋白表达的比例高于恶性组织。体内和体外研究中hNIS表达差异的原因有待进一步研究。
