Evaluation of laboratory techniques for differentiation between Entamoeba histolytica and Entamoeba dispar.

Incorporation of clinical, epidemiological and serological parameters to the studies of Entamoeba histolytica resulted in the conclusion that species complex comprising two morphologically identical amoeba were implicated with the disease. The two organisms are E. histolytica and E. dispar. The former is a pathogen and responsible for invasive amoebiasis while the later is a gut commensal. Clearly accurate diagnostic tools are required to distinguish the two species. The use of a stool ELISA has been shown to be useful. A monoclonal enzyme linked immunosorbent assay based kit to detect antigen of E.histolytica only in stool was evaluated in comparison to the results of the microscopical examination of the stools and that of enzyme linked immunosorbent assay used to detect the anti- E. histolytica IgG in serum. This study demonstrated that E. dispar is prevalent in the community and offers promise for E. histolytica monoclonal enzyme immunoassay for the qualitative and semiquantitative determination of sensu lato antigen in stool as a sensitive tool for detection and distinction of E. histolytica from E. dispar infections.