Helmy Moshira M F, Rashed Laila A, Abdel-Fattah Hisham S
Department of Parasitology, Faculty of Medicine, MISR University for Science and Technology, Egypt.
J Egypt Soc Parasitol. 2007 Apr;37(1):257-74.
A total of 140 out of 180 outpatients attended MISR University for Science and Technology Hospital complained of abdominal pain, diarrhoea and/or dysentery. Stool examination showed 47 (33.6%) had Entamoeba sp., 36 (25.7%) had cysts and 11 (7.9%) had trophozoites. Of 40 asymptomatic ones, 4 (10%) had cysts. A total of 51 positive stool samples for Entamoeba sp. (40 cysts & 11 trophozoites) were tested by Ne-sted Polymerase Chain Reaction (N-PCR) and Restriction Enzyme Digestion (RED) to clarify true E. histolytica from E. dispar. The results showed that 9/51 (17.6%) had E. dispar, while 31 (60.8%) had E. histolytica and 11 (21.6%) had dual infection with both E. histolytica and E. dispar. All E. histolytica PCR proved cases were from the symptomatic group, 11 had trophozoites and 34 had cysts. Thus, the result showed the potential use of molecular tools in detection of E. histolytica and E. dispar, and is a promising tool for epidemiology, particularly to differentiate pathogenic and non pathogenic Entamoeba sp.
在米斯尔科技大学医院就诊的180名门诊患者中，共有140人主诉腹痛、腹泻和/或痢疾。粪便检查显示，47人（33.6%）感染了内阿米巴属，36人（25.7%）有包囊，11人（7.9%）有滋养体。在40名无症状者中，4人（10%）有包囊。对51份内阿米巴属粪便阳性样本（40份包囊和11份滋养体）进行巢式聚合酶链反应（N-PCR）和限制性酶切（RED）检测，以区分溶组织内阿米巴和迪斯帕内阿米巴。结果显示，51份样本中有9份（17.6%）感染了迪斯帕内阿米巴，31份（60.8%）感染了溶组织内阿米巴，11份（21.6%）同时感染了溶组织内阿米巴和迪斯帕内阿米巴。所有经PCR证实的溶组织内阿米巴病例均来自有症状组，其中11例有滋养体，34例有包囊。因此，结果表明分子工具在检测溶组织内阿米巴和迪斯帕内阿米巴方面具有潜在用途，是流行病学研究的一种有前景的工具，特别是在区分致病性和非致病性内阿米巴属方面。
