Su W C, Shiesh S C, Liu H S, Chen C Y, Chow N H, Lin X Z
Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Dig Dis Sci. 2001 Jul;46(7):1387-92. doi: 10.1023/a:1010619316436.
The expression of several growth factors and K-ras gene mutation in bile were studied to better understand the pathogenesis and improve early diagnosis of bile duct cancers. Bile samples were collected from 12 cholangiocarcinomas (CLC), 10 ampullary cancers (APC), 3 gallbladder cancers (GBC), 7 pancreatic cancers (PNC), 9 biliary tract infection (BTI), 8 biliary stone disease (ST), and 5 normal controls (NC). The highest mean value of TGF-beta in bile was in patients with BTI; the mean levels of bFGF and PDGF were highest in CLC, and patients with APC and CLC had higher expression of HER2/Neu than other groups. In bile, a K-ras gene codon 12 mutation was found in 5 of 6 (83%) cases of CLC by the PCR-RFLP method. The results suggest overexpression of bFGF, PDGF, and HER2/Neu and the presence of K-ras mutation are important for carcinogenesis of bile duct cancers, and detection of the above abnormalities in bile is helpful for early diagnosis.
研究胆汁中几种生长因子的表达及K-ras基因突变，以更好地理解胆管癌的发病机制并改善其早期诊断。收集了12例胆管癌（CLC）、10例壶腹癌（APC）、3例胆囊癌（GBC）、7例胰腺癌（PNC）、9例胆道感染（BTI）、8例胆石症（ST）患者的胆汁样本以及5例正常对照（NC）的胆汁样本。胆汁中转化生长因子-β（TGF-β）的平均最高值出现在BTI患者中；碱性成纤维细胞生长因子（bFGF）和血小板衍生生长因子（PDGF）的平均水平在CLC患者中最高，APC和CLC患者中HER2/Neu的表达高于其他组。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，在6例CLC患者中的5例（83%）胆汁样本中发现了K-ras基因密码子12突变。结果表明，bFGF、PDGF和HER2/Neu的过表达以及K-ras突变的存在对胆管癌的致癌作用很重要，检测胆汁中的上述异常有助于早期诊断。
