Gachon F, Gaudray G, Thébault S, Basbous J, Koffi J A, Devaux C, Mesnard J
Laboratoire Infections Rétrovirales et Signalisation Cellulaire, CNRS EP 2104/Université Montpellier I, Institut de Biologie, 4 Bd Henri IV, 34060 Montpellier, France.
FEBS Lett. 2001 Jul 27;502(1-2):57-62. doi: 10.1016/s0014-5793(01)02646-1.
cAMP response element binding protein-2 (CREB-2) is a basic leucine zipper (bZIP) factor that was originally described as a repressor of CRE-dependent transcription but that can also act as a transcriptional activator. Moreover, CREB-2 is able to function in association with the viral Tax protein as an activator of the human T-cell leukemia virus type I (HTLV-I) promoter. Here we show that CREB-2 is able to interact with C/EBP-homologous protein (CHOP), a bZIP transcription factor known to inhibit CAAT/enhancer-dependent transcription. Cotransfection of CHOP with CREB-2 results in decreased activation driven by the cellular CRE motif or the HTLV-I proximal Tax-responsive element, confirming that CREB-2 and CHOP can interact with each other in vivo.
环磷酸腺苷反应元件结合蛋白2（CREB - 2）是一种碱性亮氨酸拉链（bZIP）因子，最初被描述为CRE依赖性转录的抑制因子，但也可作为转录激活因子发挥作用。此外，CREB - 2能够与病毒Tax蛋白协同作用，作为人类I型T细胞白血病病毒（HTLV - I）启动子的激活因子。在此我们表明，CREB - 2能够与C/EBP同源蛋白（CHOP）相互作用，CHOP是一种已知可抑制CAAT/增强子依赖性转录的bZIP转录因子。CHOP与CREB - 2共转染导致由细胞CRE基序或HTLV - I近端Tax反应元件驱动的激活作用降低，证实CREB - 2和CHOP在体内能够相互作用。
