关于分离的黄化质体叶绿素（酯）合成的一些观察
Some observations on chlorophyll(ide) synthesis by isolated etioplasts.
作者信息
Griffiths W T
出版信息
Biochem J. 1975 Jan;146(1):17-24. doi: 10.1042/bj1460017.
Abstract
- A modified procedure for the isolation of etioplasts from dark-grown barley is described and the regeneration of phototransformable protchlorophyll(ide) was demonstrated in the isolated plastids. 2. On exposure of the etioplasts to a long-term flash illumination, chlorophyll(ide) synthesis from a precursor pool, which includes all the protochlorophyllide, was demonstrated. 3. Added delta-aminolaevulinic acid failed to be significantly incorporated into chlorophyll(ide) in the etioplasts despite its extensive incorporation into porphyrin precursors of chlorophyll and haem compounds. The findings are discussed in terms of the inability of etioplasts to carry out the metal-insertion step in chlorophyll synthesis. 4. An elevated chlorophyll(ide) concentration was attained in the etioplasts by increasing the size of the utilizable precursor pool by pre-feeding whole plants with delta-aminolaevulinic acid, isolating the etioplasts and subjecting them to the flash illumination.
摘要
- 描述了一种从黑暗生长的大麦中分离黄化质体的改良方法，并在分离的质体中证明了光可转化原叶绿素（酸）的再生。2. 黄化质体经长期闪光照射后，证明了从前体库合成叶绿素（酸），该前体库包括所有原叶绿素酸。3. 尽管δ-氨基乙酰丙酸大量掺入叶绿素和血红素化合物的卟啉前体中，但在黄化质体中添加的δ-氨基乙酰丙酸未能显著掺入叶绿素（酸）中。根据黄化质体无法进行叶绿素合成中的金属插入步骤来讨论这些发现。4. 通过用δ-氨基乙酰丙酸预喂整株植物、分离黄化质体并使其接受闪光照射来增加可利用前体库的大小，从而在黄化质体中获得了升高的叶绿素（酸）浓度。
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