黄化质体膜制剂中叶绿素（叶绿素酸酯）合成终末阶段的特性分析。
Characterization of the terminal stages of chlorophyll (ide) synthesis in etioplast membrane preparations.
作者信息
Griffiths W T
出版信息
Biochem J. 1975 Dec;152(3):623-35. doi: 10.1042/bj1520623.
Abstract
- Chlorophyll (ide) formation from protochlorophyll (ide) that is normally inactive was demonstrated in etioplast membranes isolated from maize and barlley plants, the process being dependent on intermittent illumination and the addition of NADPH. 2. The addition of NADPH to the membranes was shown to result in the conversion of inactive protochlorophyll (ide) absorbing at about 630 nm into a form(s) with light-absorption maxima at about 640 and 652 nm, both of which disappear when chlorophyll (ide) is formed on illumination. 3. The temperature-dependence of the activation process and its response to a variety of reagents were examined. From these, the conclusion is drawn that -SH groups are involved in the activation but in the active complex these are unavailable for reaction with -SH reagents. 4. Evidence is presented for the occurrence of glucose 6-phosphate dehydrogenase activity within etioplasts and the suggestion is made that the oxidative pentose phosphate pathway can provide the NADPH required for chlorophyll biosynthesis during the early stages of greening.
摘要
- 在从玉米和大麦植株分离出的黄化质体膜中，证明了由通常无活性的原叶绿素（酯）形成叶绿素（酯），该过程依赖于间歇光照和添加NADPH。2. 已表明向膜中添加NADPH会导致在约630nm处吸收的无活性原叶绿素（酯）转化为在约640和652nm处具有最大光吸收的一种或多种形式，在光照下形成叶绿素（酯）时，这两种形式都会消失。3. 研究了激活过程的温度依赖性及其对各种试剂的反应。由此得出结论，-SH基团参与激活，但在活性复合物中，这些基团无法与-SH试剂反应。4. 提供了黄化质体内存在6-磷酸葡萄糖脱氢酶活性的证据，并提出氧化戊糖磷酸途径可以在绿化早期阶段提供叶绿素生物合成所需的NADPH。
相似文献
1
Characterization of the terminal stages of chlorophyll (ide) synthesis in etioplast membrane preparations.黄化质体膜制剂中叶绿素（叶绿素酸酯）合成终末阶段的特性分析。
Biochem J. 1975 Dec;152(3):623-35. doi: 10.1042/bj1520623.
2
Biosynthesis of chlorophyll from protochlorophyll(ide) in green plant leaves.绿色植物叶片中叶绿素从原叶绿素（酯）的生物合成。
Biochemistry (Mosc). 2002 Aug;67(8):949-55. doi: 10.1023/a:1019983124484.
3
Protochlorophyllide phototransformation in the bundle sheath cells of Zea mays.玉米维管束鞘细胞中原叶绿素酸酯的光转化
J Photochem Photobiol B. 2004 Jul 19;75(1-2):73-80. doi: 10.1016/j.jphotobiol.2004.05.008.
4
Monogalactosyldiacylglycerol Facilitates Synthesis of Photoactive Protochlorophyllide in Etioplasts.单半乳糖基二酰基甘油促进黄化质体中光活性原叶绿素酸酯的合成。
Plant Physiol. 2017 Aug;174(4):2183-2198. doi: 10.1104/pp.17.00304. Epub 2017 Jun 27.
5
Regulation of etioplast pigment-protein complexes, inner membrane architecture, and protochlorophyllide a chemical heterogeneity by light-dependent NADPH:protochlorophyllide oxidoreductases A and B.光依赖型NADPH：原叶绿素酸酯氧化还原酶A和B对黄化质体色素 - 蛋白质复合物、内膜结构及原叶绿素酸酯a化学异质性的调控
Plant Physiol. 2000 Dec;124(4):1678-96. doi: 10.1104/pp.124.4.1678.
6
Chloroplast biogenesis. Detection of monovinyl protochlorophyll(ide) b in plants.叶绿体生物发生。植物中单乙烯基原叶绿素（酯）b的检测。
J Biol Chem. 1991 Sep 15;266(26):17151-7.
7
Reconstitution of chlorophyllide formation by isolated etioplast membranes.分离的黄化质体膜对叶绿素酸酯形成的重建
Biochem J. 1978 Sep 15;174(3):681-92. doi: 10.1042/bj1740681.
8
Comparison of Light-dependent Oxygen Uptake, Protochlorophyll(ide)-650 Photoconversion, and Chlorophyll Disappearance in Wheat Etioplasts.小麦黄化质体中光依赖型氧气摄取、原叶绿素（酸酯）-650光转化及叶绿素消失的比较
Plant Physiol. 1978 Jun;61(6):1006-9. doi: 10.1104/pp.61.6.1006.
9
Etioplasts with protochlorophyll and protochlorophyllide forms in the under-soil epicotyl segments of pea (Pisum sativum) seedlings grown under natural light conditions.在自然光照条件下生长的豌豆（Pisum sativum）幼苗地下上胚轴段中存在前叶绿素和原叶绿酸形式的前质体。
Physiol Plant. 2013 Jun;148(2):307-15. doi: 10.1111/j.1399-3054.2012.01714.x. Epub 2012 Nov 21.
10
Protochlorophyll and protochlorophyllide as precursors for chlorophyll synthesis in vitro.原叶绿素和原叶绿素酸酯作为体外叶绿素合成的前体。
FEBS Lett. 1974 Dec 15;49(2):196-200. doi: 10.1016/0014-5793(74)80510-7.
引用本文的文献
1
Light dependent protochlorophyllide oxidoreductase: a succinct look.光依赖型原叶绿素酸酯氧化还原酶：简要概述。
Physiol Mol Biol Plants. 2024 May;30(5):719-731. doi: 10.1007/s12298-024-01454-5. Epub 2024 May 29.
2
NADPH:protochlorophyllide oxidoreductase B (PORB) action in Arabidopsis thaliana revisited through transgenic expression of engineered barley PORB mutant proteins.通过转化表达工程化大麦 PORB 突变体蛋白，重新研究拟南芥中的 NADPH：原叶绿素酸氧化还原酶 B（PORB）的作用。
Plant Mol Biol. 2017 May;94(1-2):45-59. doi: 10.1007/s11103-017-0592-x. Epub 2017 Mar 4.
3
Effect of cadmium on growth, bacoside A, and bacopaside I of Bacopa monnieri (L.), a memory enhancing herb.镉对作为一种增强记忆的草药的水蕹菜的生长、总内酯A和假马齿苋皂苷I的影响。
ScientificWorldJournal. 2014 Jan 30;2014:824586. doi: 10.1155/2014/824586. eCollection 2014.
4
Phototransformation of protochlorophyllideF657 in etiochloroplasts isolated from pine cotyledons; dark reformation of this pigment-complex from a pool of ALA-protochlorophyllideF635 in the presence of NADPH.从松树子叶分离出的黄化叶绿体中原叶绿素酸酯F657的光转化；在NADPH存在下，由δ-氨基乙酰丙酸-原叶绿素酸酯F635库对该色素复合物进行的暗转化。
Photosynth Res. 1980 Jun;1(2):105-13. doi: 10.1007/BF00018227.
5
Chloroplast development and the synthesis of chlorophyll a and b and chlorophyll protein complexes I and II in the dark in Tradescantia albiflora (Kunth).白花紫露草叶绿体发育及叶绿素 a、b 和叶绿素蛋白复合物 I、II 的暗合成。
Planta. 1980 Dec;150(4):269-74. doi: 10.1007/BF00384654.
6
Light-regulated pigment interconversion in pheophytin/chlorophyll-containing complexes formed during plant leaves greening.在植物叶片变绿过程中形成的含有叶绿素和脱镁叶绿素的复合物中，光调控的色素互变。
Photosynth Res. 1995 Jan;46(3):445-53. doi: 10.1007/BF00032299.
7
The NADPH-protochlorophyllide oxidoreductase is the major protein constituent of prolamellar bodies in wheat (Triticum aestivum L.).NADPH-原叶绿素氧化还原酶是小麦（Triticum aestivum L.）前质体中的主要蛋白质组成部分。
Planta. 1985 Jun;164(3):396-9. doi: 10.1007/BF00402952.
8
One divinyl reductase reduces the 8-vinyl groups in various intermediates of chlorophyll biosynthesis in a given higher plant species, but the isozyme differs between species.一种二烯还原酶在特定高等植物物种的叶绿素生物合成的各种中间产物中还原 8-乙烯基，但同工酶在物种间存在差异。
Plant Physiol. 2013 Jan;161(1):521-34. doi: 10.1104/pp.112.208421. Epub 2012 Nov 15.
9
Dominance of a 675 nm chlorophyll(ide) form upon selective 632.8 or 654 nm laser illumination after partial protochlorophyllide phototransformation.在部分原叶绿素光转化后，用选择性 632.8nm 或 654nm 激光照射时，会出现 675nm 叶绿素（原卟啉）形式的优势。
Photosynth Res. 2012 Dec;114(2):111-20. doi: 10.1007/s11120-012-9782-1. Epub 2012 Oct 28.
10
Early and late plastid development in response to chill stress and heat stress in wheat seedlings.小麦幼苗响应冷胁迫和热胁迫的早期和晚期质体发育。
Protoplasma. 2011 Oct;248(4):725-36. doi: 10.1007/s00709-010-0235-4. Epub 2010 Nov 10.
本文引用的文献
1
The control of photosynthetic carbon metabolism.光合碳代谢的调控
Science. 1971 May 7;172(3983):526-34. doi: 10.1126/science.172.3983.526.
2
Regulation of glucose 6-phosphate dehydrogenase in blue-green algae.蓝藻中葡萄糖-6-磷酸脱氢酶的调控
Plant Physiol. 1975 Apr;55(4):658-62. doi: 10.1104/pp.55.4.658.
3
Effect of Disalicylidenepropanediamine on the Light-dependent Reduction of Carbon Dioxide and Glycerate 3-Phosphate in Intact Spinach Chloroplasts.二水杨醛缩丙二胺对完整菠菜叶绿体中二氧化碳和甘油酸 3-磷酸光依赖性还原的影响。
Plant Physiol. 1975 Jan;55(1):12-4. doi: 10.1104/pp.55.1.12.
4
Properties of Protochlorophyllide and Chlorophyll(ide) Holochromes from Etiolated and Greening Leaves.原卟啉和叶绿素（ide）的性质从黄化和绿化叶片。
Plant Physiol. 1974 Mar;53(3):419-25. doi: 10.1104/pp.53.3.419.
5
Induction of porphyrin synthesis in etiolated bean leaves by chelators of iron.铁螯合剂对黄化菜豆叶片卟啉合成的诱导作用
Plant Physiol. 1974 Feb;53(2):206-15. doi: 10.1104/pp.53.2.206.
6
A Reversible Conversion of Phototransformable Protochlorophyll(ide)(656) to Photoinactive Protochlorophyll(ide)(656) by Hydrogen Sulfide in Etiolated Bean Leaves.在黄化菜豆叶片中，硫化氢介导的光可转化原叶绿素（ide）（656）向光失活原叶绿素（ide）（656）的可逆转变
Plant Physiol. 1973 Jan;51(1):139-45. doi: 10.1104/pp.51.1.139.
7
Chlorophyll Formation in Greening Bean Leaves during the Early Stages.绿豆叶片早期绿化过程中的叶绿素形成
Plant Physiol. 1973 Jan;51(1):115-9. doi: 10.1104/pp.51.1.115.
8
The Correlated Appearance of Prolamellar Bodies, Protochlorophyll(ide) Species, and the Shibata Shift during Development of Bean Etioplasts in the Dark.黑暗条件下培养的菜豆质体中前质体、原叶绿素（体）物种和 Shibata 位移的相关性出现。
Plant Physiol. 1972 Apr;49(4):619-26. doi: 10.1104/pp.49.4.619.
9
Relationship between Photoconvertible and Nonphotoconvertible Protochlorophyllides.可光转化和非可光转化原叶绿素之间的关系。
Plant Physiol. 1971 Oct;48(4):383-8. doi: 10.1104/pp.48.4.383.
10
Rapid regeneration of protochlorophyllide(650).原叶绿素酸酯（650）的快速再生
Plant Physiol. 1970 Feb;45(2):201-5. doi: 10.1104/pp.45.2.201.
Zotero 插件