Barbiroli B, Tadolini B, Moruzzi M S, Monti M G
Biochem J. 1975 Mar;146(3):687-96. doi: 10.1042/bj1460687.
Nuclei from liver of rats accustomed to eating during the first 8h of a daily 12h dark period demonstrate an increased capacity to synthesize RNA 6H after the beginning of the feeding period. 2. This increase is accompanied by a higher yield of extractable form-B DNA-dependent RNA polymerase activity. 3. The endogenous RNA polymerase activity associated with nuclear chromatin is also stimulated by food intake. Both purified and chromatin-associated form-B enzyme activities exhibit different ionic strength requirements after food intake. 4. The sensitivity of exogenous (added) form-B-enzyme to changes in ionic strength changes after feeding when chromatin is used as template. 5. Chromatin extracted from the liver of fed rats is a better template for form-B-enzyme than chromatin extracted from starved rats.
习惯于在每日12小时黑暗期的前8小时进食的大鼠肝脏细胞核,在进食期开始6小时后显示出合成RNA的能力增强。2. 这种增加伴随着可提取的B型DNA依赖性RNA聚合酶活性产量的提高。3. 与核染色质相关的内源性RNA聚合酶活性也受到食物摄入的刺激。进食后,纯化的和与染色质相关的B型酶活性都表现出不同的离子强度要求。4. 当使用染色质作为模板时,进食后外源(添加的)B型酶对离子强度变化的敏感性发生改变。5. 从喂食大鼠肝脏中提取的染色质比从饥饿大鼠肝脏中提取的染色质是B型酶更好的模板。