信使核糖核蛋白在HeLa细胞中的代谢稳定性

Metabolic stability of messenger ribonucleoprotein in HeLa cells.

作者信息

Auerbach S, Pederson T

出版信息

Biochim Biophys Acta. 1975 Jul 7;395(3):388-91. doi: 10.1016/0005-2787(75)90211-7.

Abstract

The proteins bound to HeLa cell polyribosomal messenger RNA were isolated by subjecting salt-washed, puromycin-disassembled polyribosomes to a limited digestion with pancreatic ribonuclease (ref. 1, Auerbach, S. and Pederson, T. (1975) Biochem. Biophys. Res. Commun. 63, 149-153). Label-chase experiments with radioactive amino acids revealed that the in vivo decay kinetics of the messenger RNA-associated proteins were approximately first-order, with t1/2 equal 13-15 h. The results suggest that HeLa messenger RNA and its specific set of associated proteins do not behave as single units metabolically.

摘要

通过用胰核糖核酸酶对盐洗过的、经嘌呤霉素拆解的多核糖体进行有限消化，分离出与海拉细胞多核糖体信使核糖核酸结合的蛋白质（参考文献1，奥尔巴赫，S.和佩德森，T.（1975年）《生物化学与生物物理学研究通讯》63，149 - 153）。用放射性氨基酸进行的标记追踪实验表明，信使核糖核酸相关蛋白质在体内的衰变动力学近似为一级反应，半衰期为13 - 15小时。结果表明，海拉信使核糖核酸及其特定的一组相关蛋白质在代谢上并非作为单一单元发挥作用。