信使核糖核酸相关蛋白可能参与蛋白质合成。
Possible involvement of messenger RNA-associated proteins in protein synthesis.
作者信息
Schmid H P, Köhler K, Setyono B
出版信息
J Cell Biol. 1982 Jun;93(3):893-8. doi: 10.1083/jcb.93.3.893.
Abstract
Two distinct forms of globin messenger RNA were isolated from mouse spleen cells infected with Friend erythroleukemia virus: polyribosomal messenger ribonucleoprotein particles (15S mRNP), and their corresponding protein-free mRNAs obtained by chemical deproteinization. The translation efficiencies of both messenger forms were assayed in a Krebs II ascites cell-free system. Selective removal of RNA-binding proteins from the ascites cell lysate did not affect globin synthesis when the mRNA was supplied as 15S mRNP; deproteinized mRNA however was not translated. Only in the presence of two fractions of RNA-binding proteins was the protein-free mRNA translated. Some of the RNA-binding proteins have the same molecular weights and isoelectric points as the principal proteins of 15S mRNP.
摘要
从小鼠脾脏细胞中分离出两种不同形式的珠蛋白信使核糖核酸（mRNA），这些细胞感染了弗瑞德白血病病毒：多核糖体信使核糖核蛋白颗粒（15S mRNP），以及通过化学脱蛋白获得的相应无蛋白mRNA。在克雷布斯II腹水无细胞系统中测定了两种信使形式的翻译效率。当mRNA以15S mRNP形式提供时，从腹水细胞裂解物中选择性去除RNA结合蛋白并不影响珠蛋白合成；然而，脱蛋白的mRNA未被翻译。只有在存在两部分RNA结合蛋白的情况下，无蛋白mRNA才会被翻译。一些RNA结合蛋白的分子量和等电点与15S mRNP的主要蛋白相同。
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本文引用的文献
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Nucleic Acids Res. 1980 Dec 11;8(23):5685-701. doi: 10.1093/nar/8.23.5685.
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Cross-linking of mRNA to proteins by irradiation of intact cells with ultraviolet light.通过用紫外线照射完整细胞使信使核糖核酸与蛋白质交联。
Eur J Biochem. 1980 Nov;112(2):323-30. doi: 10.1111/j.1432-1033.1980.tb07207.x.
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Proteins associated with poly(A) and other regions of mRNA and hnRNA molecules as investigated by crosslinking.通过交联研究与mRNA和hnRNA分子的多聚腺苷酸（poly(A)）及其他区域相关的蛋白质。
Cell. 1981 Jun;24(3):775-83. doi: 10.1016/0092-8674(81)90103-3.
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The polyribosomal mRNA--protein complex is a dynamic structure.多核糖体信使核糖核酸-蛋白质复合体是一种动态结构。
Proc Natl Acad Sci U S A. 1981 May;78(5):2923-6. doi: 10.1073/pnas.78.5.2923.
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