Schmid H P, Köhler K, Setyono B
J Cell Biol. 1982 Jun;93(3):893-8. doi: 10.1083/jcb.93.3.893.
Two distinct forms of globin messenger RNA were isolated from mouse spleen cells infected with Friend erythroleukemia virus: polyribosomal messenger ribonucleoprotein particles (15S mRNP), and their corresponding protein-free mRNAs obtained by chemical deproteinization. The translation efficiencies of both messenger forms were assayed in a Krebs II ascites cell-free system. Selective removal of RNA-binding proteins from the ascites cell lysate did not affect globin synthesis when the mRNA was supplied as 15S mRNP; deproteinized mRNA however was not translated. Only in the presence of two fractions of RNA-binding proteins was the protein-free mRNA translated. Some of the RNA-binding proteins have the same molecular weights and isoelectric points as the principal proteins of 15S mRNP.
从小鼠脾脏细胞中分离出两种不同形式的珠蛋白信使核糖核酸（mRNA），这些细胞感染了弗瑞德白血病病毒：多核糖体信使核糖核蛋白颗粒（15S mRNP），以及通过化学脱蛋白获得的相应无蛋白mRNA。在克雷布斯II腹水无细胞系统中测定了两种信使形式的翻译效率。当mRNA以15S mRNP形式提供时，从腹水细胞裂解物中选择性去除RNA结合蛋白并不影响珠蛋白合成；然而，脱蛋白的mRNA未被翻译。只有在存在两部分RNA结合蛋白的情况下，无蛋白mRNA才会被翻译。一些RNA结合蛋白的分子量和等电点与15S mRNP的主要蛋白相同。
