复杂基因组系统测序的策略。

Strategies for the systematic sequencing of complex genomes.

作者信息

Green E D

机构信息

Genome Technology Branch and NIH Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Nat Rev Genet. 2001 Aug;2(8):573-83. doi: 10.1038/35084503.

DOI:10.1038/35084503

PMID:11483982

Abstract

Recent spectacular advances in the technologies and strategies for DNA sequencing have profoundly accelerated the detailed analysis of genomes from myriad organisms. The past few years alone have seen the publication of near-complete or draft versions of the genome sequence of several well-studied, multicellular organisms - most notably, the human. As well as providing data of fundamental biological significance, these landmark accomplishments have yielded important strategic insights that are guiding current and future genome-sequencing projects.

摘要

近年来，DNA测序技术和策略取得了惊人进展，极大地加速了对众多生物体基因组的详细分析。仅在过去几年里，就有几种经过充分研究的多细胞生物体的基因组序列近乎完整版本或草图得以发表，其中最引人注目的是人类基因组。这些具有里程碑意义的成果不仅提供了具有重要生物学意义的数据，还产生了重要的战略见解，为当前和未来的基因组测序项目提供了指导。