Chl12（Ctf18）形成一种新型的复制因子C相关复合物，并在DNA复制检查点途径中与Rad24发挥冗余功能。
Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway.
作者信息
Naiki T, Kondo T, Nakada D, Matsumoto K, Sugimoto K
机构信息
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-0814, Japan.
出版信息
Mol Cell Biol. 2001 Sep;21(17):5838-45. doi: 10.1128/MCB.21.17.5838-5845.2001.
RAD24 has been identified as a gene essential for the DNA damage checkpoint in budding yeast. Rad24 is structurally related to subunits of the replication factor C (RFC) complex, and forms an RFC-related complex with Rfc2, Rfc3, Rfc4, and Rfc5. The rad24Delta mutation enhances the defect of rfc5-1 in the DNA replication block checkpoint, implicating RAD24 in this checkpoint. CHL12 (also called CTF18) encodes a protein that is structurally related to the Rad24 and RFC proteins. We show here that although neither chl12Delta nor rad24Delta single mutants are defective, chl12Delta rad24Delta double mutants become defective in the replication block checkpoint. We also show that Chl12 interacts physically with Rfc2, Rfc3, Rfc4, and Rfc5 and forms an RFC-related complex which is distinct from the RFC and RAD24 complexes. Our results suggest that Chl12 forms a novel RFC-related complex and functions redundantly with Rad24 in the DNA replication block checkpoint.
RAD24已被确定为芽殖酵母中DNA损伤检查点所必需的基因。Rad24在结构上与复制因子C（RFC）复合体的亚基相关，并与Rfc2、Rfc3、Rfc4和Rfc5形成一个RFC相关复合体。rad24Delta突变增强了rfc5-1在DNA复制阻断检查点中的缺陷，表明RAD24参与了该检查点。CHL12（也称为CTF18）编码一种在结构上与Rad24和RFC蛋白相关的蛋白质。我们在此表明，尽管chl12Delta和rad24Delta单突变体均无缺陷，但chl12Delta rad24Delta双突变体在复制阻断检查点中出现缺陷。我们还表明，Chl12与Rfc2、Rfc3、Rfc4和Rfc5发生物理相互作用，并形成一个与RFC和RAD24复合体不同的RFC相关复合体。我们的结果表明，Chl12形成一个新的RFC相关复合体，并在DNA复制阻断检查点中与Rad24发挥冗余功能。
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