Pai H H, Liu C S, Chen E R
Graduate Institute of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan.
Kaohsiung J Med Sci. 2001 Mar;17(3):120-5.
In order to clarify growth effects of gamma-ray irradiation on the third-stage larvae of Angiostrongylus cantonensis, snails naturally infected with larvae of A. cantonensis were irradiated with a cobalt-60 source, and the total dosage of exposure was 0.1 kGy. Third-stage larvae were collected from each group of irradiated and non-irradiated snails and then administered to rats. A significant reduction was observed in the number of recovery worms from rats sacrificed at 45 days after infection compared with the control group. Moreover, there was an increase in the percentage of female worms, and the body length of the irradiated group's worms was significantly shorter than that of the control group. In ultrastructural observations by transmission electron microscope, the cuticle was thickened and the subcuticle showed partial vacuolization and separation from muscle in the irradiated group. In addition, the muscles myofibril became shorter, the microvilli of intestinal mucosa were shorter and irregular, the terminal web disappeared, and the phagocytic vesicles, mitochondria, lysosomes and secretion granules decreased in number, respectively. Focusing on the mitochondria of the epithelial cells of the intestine, there were degenerative changes in the irradiated group showing vacuolization. The gonads of female worms recovered from the irradiated group revealed degenerative vacuolization, and the gonads of male worms could not be found.
为阐明γ射线辐照对广州管圆线虫三期幼虫的生长影响，用钴-60源对自然感染广州管圆线虫幼虫的螺进行辐照，总照射剂量为0.1千戈瑞。从每组辐照和未辐照的螺中收集三期幼虫，然后接种给大鼠。与对照组相比，感染后45天处死的大鼠体内回收虫体数量显著减少。此外，雌虫比例增加，辐照组虫体的体长显著短于对照组。在透射电子显微镜的超微结构观察中，辐照组的角质层增厚，皮下组织出现部分空泡化并与肌肉分离。此外，肌肉肌原纤维变短，肠黏膜微绒毛变短且不规则，终末网消失，吞噬泡、线粒体、溶酶体和分泌颗粒数量分别减少。聚焦于肠上皮细胞的线粒体，辐照组出现空泡化的退行性变化。从辐照组回收的雌虫性腺呈现退行性空泡化，未发现雄虫性腺。
