Pai H H, Ko Y C, Chen E R
Graduate Institute of Medicine, Kaohsiung Medical College, Taiwan, Republic of China.
Am J Trop Med Hyg. 1993 Jun;48(6):827-30. doi: 10.4269/ajtmh.1993.48.827.
Cobalt-60 gamma irradiation was used to kill the larvae of Angiostrongylus cantonensis in four groups of snails that were irradiated at a rate of 15.4 Gy/min for 16.23 min (0.25 kGy), 32.47 min (0.50 kGy), 48.70 min (0.75 kGy), and 64.94 min (1.00 kGy), respectively. Third-stage (infected stage) larvae were isolated from these snails and 50 were administered to each experimental animal via an esophageal tube. At a dosage of 0.25 kGy, the mean +/- SD number of worms recovered from 30 rats and 30 mice was 15.6 +/- 6.5 and 11.3 +/- 6.2, respectively. These were significantly different from the number of worms recovered from the nonirradiated (control) groups, which were 28.9 +/- 9.6 (P < 0.05) and 16.6 +/- 10.3 (P < 0.05) for rats and mice respectively. Worms and cellular infiltrations in lung tissue of rats were histopathologically identified in the 0.25-kGy-irradiated group as well as in the control group. Worms and eosinophilic meningitis were also noted in the brain tissue of mice in control groups. No worms or pathologic findings were observed in the heart, lung, or brain tissue of rats or mice infected with larvae irradiated at or exceeding 0.50 kGy. It is therefore concluded that a dosage of 0.50 kGy is sufficient to prevent successful infection in mice and rats.
采用钴 - 60γ射线辐照，以15.4 Gy/分钟的速率分别对四组蜗牛进行辐照，辐照时间分别为16.23分钟（0.25 kGy）、32.47分钟（0.50 kGy）、48.70分钟（0.75 kGy）和64.94分钟（1.00 kGy），以杀死广州管圆线虫幼虫。从这些蜗牛中分离出第三期（感染期）幼虫，通过食管管给每只实验动物投喂50条。在0.25 kGy的剂量下，从30只大鼠和30只小鼠体内回收的蠕虫平均数量±标准差分别为15.6±6.5和11.3±6.2。这些数值与未辐照（对照）组回收的蠕虫数量有显著差异，大鼠对照组为28.9±9.6（P < 0.05），小鼠对照组为16.6±10.3（P < 0.05）。在0.25 kGy辐照组以及对照组的大鼠肺组织中，通过组织病理学鉴定发现了蠕虫和细胞浸润。在对照组小鼠的脑组织中也发现了蠕虫和嗜酸性脑膜炎。在感染了辐照剂量达到或超过0.50 kGy幼虫的大鼠或小鼠的心脏、肺或脑组织中，未观察到蠕虫或病理变化。因此得出结论，0.50 kGy的剂量足以防止小鼠和大鼠成功感染。
