柱前衍生化高效液相色谱法测定血浆和细胞提取物中的半胱氨酸、半胱氨酰甘氨酸、同型半胱氨酸和谷胱甘肽。
Pre-column derivatization high-performance liquid chromatographic method for determination of cysteine, cysteinyl-glycine, homocysteine and glutathione in plasma and cell extracts.
作者信息
Katrusiak A E, Paterson P G, Kamencic H, Shoker A, Lyon A W
机构信息
Department of Pathology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
出版信息
J Chromatogr B Biomed Sci Appl. 2001 Jul 15;758(2):207-12. doi: 10.1016/s0378-4347(01)00182-7.
A sensitive high-performance liquid chromatographic method for quantification of sulphydryl and disulfide amino acids in human plasma using ultra violet spectrophotometric detection was developed. Precolumn derivatization with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) and an optional pre-derivatization reaction with dithiothreitol allowed both quantitative reduction of disulfides for measurement of total amino acid levels and the measurement of the reduced forms. A dynamic range of 500 nmol/l-750 micromol/l allowed the major analytes of interest to be quantified in plasma without sample dilution. The assay is a sensitive and precise method for the determination of sulphydryl and disulfide amino acids in plasma and cell extracts.
建立了一种灵敏的高效液相色谱法，用于定量测定人血浆中的巯基和二硫键氨基酸，采用紫外分光光度检测。用5,5'-二硫代双硝基苯甲酸（DTNB）进行柱前衍生，以及用二硫苏糖醇进行可选的预衍生反应，既可以对二硫键进行定量还原以测量总氨基酸水平，又可以测量还原形式。500 nmol/l - 750 μmol/l的动态范围使得无需样品稀释即可对血浆中主要的目标分析物进行定量。该测定法是一种灵敏且精确的方法，用于测定血浆和细胞提取物中的巯基和二硫键氨基酸。
Zotero 插件