Wang Wei, Chen Jiandan, Zhou Zhenzeng, Zhan Shanshan, Xing Zhiyuan, Liu Hongying, Zhang Linan
Department of Automation, Hangzhou Dianzi University, Hangzhou 310018, China.
Life (Basel). 2022 Jul 28;12(8):1142. doi: 10.3390/life12081142.
Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold-platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20-40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1-11 μmol/L, with low detection and quantification limits of 0.051 μM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection.
确定谷胱甘肽的浓度对于制定可行的医学诊断策略至关重要。在本文中，我们通过在玻碳电极表面依次电沉积氨基反应基团和金铂纳米材料，开发了一种电化学传感器。该传感器通过循环伏安法（CV）、场发射扫描电子显微镜（FESEM）、能量色散X射线光谱（EDX）和电化学阻抗谱（EIS）进行表征。结果表明，电极表面呈现出尺寸为20 - 40 nm的Au@Pt纳米颗粒。该传感器对谷胱甘肽表现出优异的电催化氧化性能。基于此，我们设计了一种用于快速灵敏检测谷胱甘肽的电化学生物传感器。在优化实验和操作条件后，获得了谷胱甘肽浓度在0.1 - 11 μmol/L范围内的线性响应，检测限和定量限低至0.051 μM（S/N = 3）。该传感器还具有优异的选择性、重现性、低成本以及制备简单的特点，可应用于人体血清样本检测。
