Ohana M, Okazaki K, Oshima C, Andra's D, Nishi T, Uchida K, Uose S, Nakase H, Matsushima Y, Chiba T
Department of Gastroenterology and Endoscopic Medicine, Faculty of Medicine, Kyoto University, 54 Shogoin-Kawara-cho, Sakyo, Kyoto, 606-8507 Japan.
Gastroenterology. 2001 Aug;121(2):329-36. doi: 10.1053/gast.2001.26289.
BACKGROUND & AIMS: Interleukin (IL)-7 is a critical cytokine in the development of T and B cells and is involved in gastrointestinal pathophysiology. The aim of this study was to investigate the involvement of IL-7 receptor (IL-7R) signaling in Helicobacter-induced gastritis.
C57BL/6 mice (n = 40) were inoculated with H. felis. Twenty mice were injected intraperitoneally with neutralizing IL-7R antibody (A7R34) every seventh day for 3 months. Histology, serum anti-H. felis antibody, and gene expression of IL-7, IL-7R, and proinflammatory cytokines in the gastric mucosa were evaluated.
Seventeen of 20 (85%) infected mice without A7R34 developed severe atrophic gastritis, whereas there was no gastritis in A7R34-treated infected mice. There was no difference in the serum levels of anti-H. felis antibody between the 2 groups. IL-7, IL-7R, IL-1alpha, tumor necrosis factor alpha, and interferon gamma messenger RNA expressions were up-regulated in control infected mice, whereas only IL-7 messenger RNA was up-regulated in A7R34-treated infected mice. Immunohistochemistry indicated positive cytoplasmic staining of IL-7 in the gastric epithelial cells.
These data suggest a critical role for IL-7 receptor signaling in the development of Helicobacter-induced gastritis in mice.
白细胞介素（IL）-7是T细胞和B细胞发育中的关键细胞因子，参与胃肠道病理生理过程。本研究旨在探讨IL-7受体（IL-7R）信号传导在幽门螺杆菌诱导的胃炎中的作用。
将40只C57BL/6小鼠接种猫幽门螺杆菌。20只小鼠每隔7天腹腔注射中和性IL-7R抗体（A7R34），共3个月。评估胃黏膜的组织学、血清抗猫幽门螺杆菌抗体以及IL-7、IL-7R和促炎细胞因子的基因表达。
20只未注射A7R34的感染小鼠中有17只（85%）发生严重萎缩性胃炎，而接受A7R34治疗的感染小鼠未出现胃炎。两组血清抗猫幽门螺杆菌抗体水平无差异。对照感染小鼠中IL-7、IL-7R、IL-1α、肿瘤坏死因子α和干扰素γ信使核糖核酸表达上调，而接受A7R34治疗的感染小鼠中仅IL-7信使核糖核酸上调。免疫组织化学显示胃上皮细胞中IL-7的细胞质染色呈阳性。
这些数据表明IL-7受体信号传导在小鼠幽门螺杆菌诱导的胃炎发生中起关键作用。
