Marona H R, Schapoval E E
Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, Rod. Araraquara-Jaú, km 1, CEP. 14801-902, SP, Araraquara, Brazil.
J Pharm Biomed Anal. 2001 Oct;26(3):501-4. doi: 10.1016/s0731-7085(01)00429-0.
A visible light spectrophotometric method is described for the determination of sparfloxacin in tablets. The procedure is based on the complexation of bromothymol blue 0.5% and sparfloxacin to form a compound of yellow colour with maximum absorption at 385 nm. The Lambert-Beer law was obeyed in the concentration range of 2-12 mg/l. The present study describes a sensitive and accurate method for the determination of the concentration of sparfloxacin in tablets. It was also found that the excipients in the commercial tablet preparation did not interfere with the assay.
本文描述了一种用于测定片剂中司帕沙星的可见光分光光度法。该方法基于0.5%溴百里酚蓝与司帕沙星络合形成黄色化合物，在385nm处有最大吸收。在2-12mg/l的浓度范围内符合朗伯-比尔定律。本研究描述了一种灵敏且准确的测定片剂中司帕沙星浓度的方法。还发现市售片剂制剂中的辅料不干扰测定。
