Wright N A, Al-Dewachi H S, Appleton D R, Watson A J
Cell Tissue Kinet. 1975 Jul;8(4):361-8. doi: 10.1111/j.1365-2184.1975.tb01500.x.
Cell kinetics in the jejunal crypt of the male Wistar rat were studied using autoradiographic techniques with tritiated thymidine and a stathmokinetic technique with vincristine. The migration rate measured by following the movement of the 50% peak on the labelling index distribution curve with time after injection of tritiated thymidine gave a value of 1-43 +/- 0-14 (SE) cell positions per hour, compared with a value from a cumulative birth rate of 1-78 cell positions per hour. Tht crypt column length was 32-9 +/- 0-2 cells and the column count was 22-3 +/- 0-2. This measurement gave a total crypt population of 734 cells, compared with an estimate of 650 +/- l from direct observation of squashed, microdissected crypts. In each crypt 22-5 +/- 0-5 mitoses were present, and the crypt cell production rate was 32 cells per crypt per hour; this latter value was confirmed using two independent techniques. The crypt growth fraction calculated from the durations of phases of the cell cycle and the labelling index was 0-62. A value of 0-61 was found from the labelling index distribution curve. As assessed from crypt squashes, there were 403 proliferating cells per crypt.
采用氚标记胸腺嘧啶核苷放射自显影技术和长春新碱有丝分裂阻断技术，对雄性Wistar大鼠空肠隐窝的细胞动力学进行了研究。通过追踪注射氚标记胸腺嘧啶核苷后标记指数分布曲线上50%峰值随时间的移动来测量迁移率，结果为每小时1.43±0.14（标准误）个细胞位置，而累积出生率得出的值为每小时1.78个细胞位置。隐窝柱长度为32.9±0.2个细胞，柱计数为22.3±0.2。该测量得出隐窝细胞总数为734个，而通过直接观察挤压、显微解剖的隐窝估计为650±1个。每个隐窝中有22.5±0.5个有丝分裂，隐窝细胞产生率为每隐窝每小时32个细胞；后一值通过两种独立技术得到证实。根据细胞周期各阶段的持续时间和标记指数计算出的隐窝生长分数为0.62。从标记指数分布曲线得出的值为0.61。从隐窝压片中评估，每个隐窝有403个增殖细胞。
