Dail W G, Evan A P, Eason H R
Cell Tissue Res. 1975 May 27;159(1):49-62. doi: 10.1007/BF00231994.
To further evaluate the role of autonomic ganglia in the regulation of pelvic visceral activity, the neural elements in the major pelvic ganglion of the male rat have been studied with histochemixal and electron microscopic techniques. The principal findings are that the ganglion is composed of cholinergic and adrenergic ganglion cells as well as small intensely fluorescent (SIF) cells. Polarity in the ganglion is indicated by clustering of small ganglion cells which stain intensely for acetylcholinesterase (AChE) along the pelvic nerve while larger cells, with weak to moderate AChE activity, collect near small branches of the hypogatrric nerve. Some cholinergic ganglion cells are enclosed by a plexus of adrenergic terminals. SIF cells appear to be in contact with both cholinergic and adrenergic cells, although many of the fluorescent beads around adrenergic neurons may be short dendrites of ganglion cells, rather than processes of SIF cells. Two types of SIF cells may be distingiosjed on the basis of size and morphology of their granulated vesicles. Afferent synapses of the cholinergic type were common on SIF cells of the large granule and small granule type. Portions of SIF cells with large granules occur within the capsule of ganglion cells. Contacts seen here were interpreted as efferent synapses from SIF cells to the dendrites of ganglion cells.
为了进一步评估自主神经节在盆腔内脏活动调节中的作用，运用组织化学和电子显微镜技术对雄性大鼠主要盆腔神经节中的神经成分进行了研究。主要发现如下：该神经节由胆碱能和肾上腺素能神经节细胞以及小而强荧光（SIF）细胞组成。神经节的极性表现为，沿盆神经分布着对乙酰胆碱酯酶（AChE）染色强烈的小神经节细胞簇，而具有弱至中度AChE活性的较大细胞则聚集在腹下神经小分支附近。一些胆碱能神经节细胞被肾上腺素能终末丛包围。SIF细胞似乎与胆碱能和肾上腺素能细胞均有接触，不过肾上腺素能神经元周围的许多荧光小体可能是神经节细胞的短树突，而非SIF细胞的突起。根据其颗粒小泡的大小和形态可区分出两种类型的SIF细胞。胆碱能型传入突触在大颗粒型和小颗粒型SIF细胞上较为常见。大颗粒SIF细胞的部分位于神经节细胞的被膜内。此处所见的接触被解释为从SIF细胞到神经节细胞树突的传出突触。
