Kusano G
Osaka University of Pharmaceutical Sciences, 4-20-1, Nasahara, Takatsuki, Osaka 569-1094, Japan.
Yakugaku Zasshi. 2001 Jul;121(7):497-521. doi: 10.1248/yakushi.121.497.
The chemical constituents of Cimicifuga simplex and its related species were reexamined using HPLC and high resolution spectral analysis. From C. simplex, C. acerina and C. japonica, a new alkaloid, 59 new cycloartane triterpene glycosides and 11 new aromatic constituents were isolated with the previously reported compounds. The latter aromatic constituents were identified in HPLC analysis of the extracts from crude drugs due to C. dahurica, C. heracleifolia and C. foetida. Several interesting topics in this study are reviewed about separation of unstable and closely related compounds by HPLC, rearrangement reactions, chemical conversion for confirmation of absolute configuration, isomerism in NMR solutions, X-ray crystal analysis, spectral analysis of complicated structures, isolation of glycoside malonates, high yield of enzymatic hydrolysis, specific CD curve due to a cycloart-7-ene system, and so on. Biological activities of a major glycoside, cimicifugoside, a modified triterpene, acerinol, and benzyltartaric acid derivatives such as fukinolic acid and cimicifugic acids were also mentioned.
使用高效液相色谱法（HPLC）和高分辨率光谱分析对单穗升麻及其近缘物种的化学成分进行了重新研究。从单穗升麻、 Acerina升麻和日本升麻中，分离出一种新生物碱、59种新的环阿尔廷烷三萜糖苷和11种新的芳香成分，以及先前报道的化合物。后几种芳香成分在对兴安升麻、独活升麻和毛叶升麻药材提取物的HPLC分析中得到鉴定。本文综述了本研究中几个有趣的主题，包括通过HPLC分离不稳定和结构相近的化合物、重排反应、用于确定绝对构型的化学转化、核磁共振溶液中的异构现象、X射线晶体分析、复杂结构的光谱分析、糖苷丙二酸酯的分离、酶促水解的高产率、环阿尔廷-7-烯系统导致的特定圆二色曲线等。还提到了主要糖苷升麻糖苷、一种修饰的三萜醇 Acerinol以及苄基酒石酸衍生物如伏牛花酸和升麻酸的生物活性。
