Sangsiriwut K, Auewarakul P, Suwanagool S, Wasi C
Department of Preventive Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 2001 Mar;19(1):43-8.
A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.
开发了一种定量竞争性巢式PCR检测法，用于定量临床样本中的HIV-1前病毒DNA。通过删除18个碱基对的序列，从HIV-1 gag基因的保守区域构建了一个竞争DNA。我们对临床样本中的HIV-1前病毒DNA拷贝数进行了定量。用该方法分析了35例CD4细胞计数范围为4-728个细胞/mm3的HIV感染患者的外周血单个核细胞（PBMC）。每10(6)个CD4+ T细胞中检测到的HIV-1 DNA拷贝数在518至67,340拷贝之间。拷贝数与CD4细胞计数呈负相关。
