Li L, Liang G, Zhao B
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2000 Sep;14(3):218-22.
To study the effect of Sindbis XJ-160 virus structural proteins on BHK-21 cell.
We constructed the expressing vector pcDNA3.1 ABC that contains the XJ-160 virus structural protein gene and then transfected it to BHK-21 cell.
The expressed products can be detected by RNA dot blot hybridization or immUnofluorescent assay. The cytopathic effect was observed in BHK-21 cells transfected with pcDNA3.1 ABC. There was also condensed chromatin found accumulated. BHK-21 cell death and marked cell cycle changes were observed.
These results indicate that cell apoptosis might be one of the mechanisms inducing cell death by XJ - 160 virus and its structural protein gene probably plays a role in this programmed cell death.
