Yman L, Ponterius G, Brandt R
Dev Biol Stand. 1975;29:151-65.
The RAST principle has been utilized for the detection and assay of allergens of various origins. RAST-based methods have the advantage of being convenient, accurate and specific by making use of the IgE antibodies, considered as mediators of the atopic allergic reactions, as reagents in vitro. Two improved assay procedures, a direct titration and an inhibition method, have been used for the estimation of extract potencies and for the assay of separate allergens. The specificity of the assays is determined by the specificity of the IgE (sera from allergic patients) used as the main reagent. Specific assay of separate components in heterogeneous extracts may be achieved in some cases by careful characterization of the sera or by selective blocking of irrelevant IgE. The potency of allergen concentration may be calculated and expressed in arbitrary units, and the accuracy and sensitivity of the two methods are similar. The coefficient of variation is in the range 10 to 25% provided that standardized reagents are used. Sensitivity of the assays is about 200 ng per ml. The lower limit for detection of allergen is 10-100 times lower.
放射性变应原吸附试验（RAST）原理已被用于检测和分析各种来源的过敏原。基于RAST的方法具有方便、准确和特异的优点，它利用被认为是特应性过敏反应介质的IgE抗体作为体外试剂。两种改进的检测程序，即直接滴定法和抑制法，已被用于评估提取物的效价和分析单独的过敏原。检测的特异性由用作主要试剂的IgE（过敏患者的血清）的特异性决定。在某些情况下，通过仔细鉴定血清或选择性阻断无关的IgE，可以实现对异质提取物中单独成分的特异性检测。过敏原浓度的效价可以计算并以任意单位表示，两种方法的准确性和灵敏度相似。如果使用标准化试剂，变异系数在10%至25%的范围内。检测的灵敏度约为每毫升200纳克。过敏原检测的下限要低10至100倍。
