Caballero T, Pascual C, García-Ara M C, Ojeda J A, Martín-Esteban M
Laboratorio de Immunoalergia, Hospital Infantil La Paz, Madrid, Spain.
Clin Exp Allergy. 1997 Oct;27(10):1203-11.
An association between sensitization to Compositae pollens and hypersensitivity to hazelnut has been previously described. There is no previous in vitro study about crossreactivity between mugwort pollen and hazelnut.
To study mugwort pollen and hazelnut allergens and to assess if there is IgE crossreactivity between mugwort pollen and hazelnut.
A serum pool formed by 28 individual sera with specific IgE to mugwort pollen and hazelnut was used to investigate IgE crossreactivity. RAST-inhibition, SDS-PAGE/IEF immunoblotting inhibition assays were performed by preincubation of the sera with mugwort pollen and hazelnut.
RAST to hazelnut was inhibited up to 63% by mugwort pollen, but the mugwort pollen RAST was only inhibited up to 36% by hazelnut. In SDS-PAGE immunoblotting mugwort pollen showed nine allergens ranging from <16 to 65 kDa and hazelnut had four main allergens: 42 kDa, 17 kDa and <16 kDa (two bands). In the SDS-PAGE immunoblotting inhibition hazelnut partially inhibited all the mugwort pollen bands, except that with 19kDa, whereas mugwort pollen produced a nearly total inhibition of all the hazelnut allergens. In isoelectrofocusing immunoblotting mugwort pollen had two groups of allergens: pI 7.5-8.5 and pI 3.5-5.2 and hazelnut one group of allergens: pI 5.2-5.8. In the isoelectrofocusing immunoblotting inhibition hazelnut produced a partial inhibition of all the bands of mugwort pollen and mugwort pollen partially inhibited all the allergenic bands of hazelnut.
The RAST and SDS-PAGE/IEF immunoblotting inhibition results provide evidence of IgE cross reactivity between mugwort pollen and hazelnut allergens. The inhibition of hazelnut by mugwort pollen is higher than the inhibition of mugwort pollen by hazelnut in both RAST inhibition and SDS-PAGE immunoblotting inhibition. These results suggest that mugwort pollen allergens would behave as primary immunogens in the association between sensitivity to mugwort pollen and hazelnut.
先前已描述过对菊科花粉的致敏与对榛子的超敏反应之间的关联。此前尚无关于艾蒿花粉与榛子之间交叉反应性的体外研究。
研究艾蒿花粉和榛子过敏原，并评估艾蒿花粉与榛子之间是否存在IgE交叉反应性。
使用由28份对艾蒿花粉和榛子具有特异性IgE的个体血清组成的混合血清来研究IgE交叉反应性。通过将血清与艾蒿花粉和榛子预孵育进行RAST抑制、SDS-PAGE/IEF免疫印迹抑制试验。
艾蒿花粉对榛子的RAST抑制率高达63%，但榛子对艾蒿花粉RAST的抑制率仅为36%。在SDS-PAGE免疫印迹中，艾蒿花粉显示出9种过敏原，分子量范围为<16至65 kDa，榛子有4种主要过敏原：42 kDa、17 kDa和<16 kDa（两条带）。在SDS-PAGE免疫印迹抑制试验中，榛子部分抑制了除19 kDa以外的所有艾蒿花粉条带，而艾蒿花粉几乎完全抑制了所有榛子过敏原。在等电聚焦免疫印迹中，艾蒿花粉有两组过敏原：pI 7.5 - 8.5和pI 3.5 - 5.2，榛子有一组过敏原：pI 5.2 - 5.8。在等电聚焦免疫印迹抑制试验中，榛子部分抑制了艾蒿花粉的所有条带，艾蒿花粉部分抑制了榛子的所有致敏条带。
RAST和SDS-PAGE/IEF免疫印迹抑制结果提供了艾蒿花粉与榛子过敏原之间IgE交叉反应性的证据。在RAST抑制和SDS-PAGE免疫印迹抑制中，艾蒿花粉对榛子的抑制高于榛子对艾蒿花粉的抑制。这些结果表明，在对艾蒿花粉和榛子敏感的关联中，艾蒿花粉过敏原可能起主要免疫原的作用。
