Dzagurov S G
Dev Biol Stand. 1975;29:261-6.
One of the prerequisites for the standardization of bacterial allergens consists in the development and formulation of uniform requirements for the assessment of the specific activity of allergens, taking into account the problems to be solved by means of these allergens: a) the screening of large groups of population in order to select those that should be vaccinated or b) the evaluation of the extent of allergic conversion in humans. The data presented here have been obtained in the Laboratory of Allergens of L.A. Tarassevich State Research Institute for Standardization and Control of Medical Biological Preparations. They show that the frequency of skin reactions depends on the concentration of protein in a single dose (e.g. with hemolytic streptococcus allergen) as well as on the properties of the bacterial strain (e.g. with staphylococcal allergens). The standardization of allergens involves the use of special in vitro reactions of blood leukocytes. In 1962 Fradkin proposed the NDI (neutrophile damage index) test based on the enhancement of the amoeboid activity of neutrophiles after the incubation of blood smears to which allergen has been added. The results of differentiation of infections and postvaccinal allergy in children by means of NDI test are discussed. It is suggested that specific forms of allergens should be manufactured for in vitro cellular reactions.
细菌变应原标准化的前提条件之一是，在考虑到借助这些变应原需解决的问题的情况下，制定并形成对变应原比活性评估的统一要求：a）对大量人群进行筛查，以挑选出应接种疫苗的人群；b）评估人体过敏转化的程度。此处呈现的数据是在LA塔拉谢维奇国家医学生物制品标准化与控制研究所变应原实验室获得的。这些数据表明，皮肤反应的频率取决于单剂量中的蛋白质浓度（例如对于溶血性链球菌变应原）以及细菌菌株的特性（例如对于葡萄球菌变应原）。变应原的标准化涉及使用血液白细胞的特殊体外反应。1962年，弗拉德金提出了NDI（中性粒细胞损伤指数）试验，该试验基于在添加了变应原的血涂片孵育后中性粒细胞阿米巴样活性的增强。讨论了通过NDI试验区分儿童感染和疫苗接种后过敏的结果。建议生产用于体外细胞反应的特定形式的变应原。
