Petrunov B, Stanoeva E
Dev Biol Stand. 1975;29:132-40.
The production and standardization of the bacterial allergens are very difficult and connected with many unknown and disputable problems. The authors have carried out a study with three basic bacterial allergens produced by themselves: bacterial extracts, bacterial suspensions and a mixture of both from Streptococcus hemolyticus and Staphylococcus albus. The specific activity of all allergens in different concentrations is determined by skin intradermal tests simultaneously on 22 patients sensitized to Str. hemolyticus. 23 patients sensitized to Staph. albus, as well as on 10 healthy persons. The results of the study show that the bacterial extracts give predominantly immediate type skin allergic reaction while the bacterial suspensions lead to delayed type reactions. The mixed allergens give both type skin reactions, and reveal more specifically sensitization against the bacteria. The authors suggest that the optimal concentration of the mixed bacterial allergens for diagnosis by intradermal test is 10,000 PNU/ml and 100,000,000 bacterial/ml. The advantages and disadvantages of the three bacterial allergens are discussed.
细菌过敏原的生产和标准化非常困难，且与许多未知和有争议的问题相关。作者对自行制备的三种基本细菌过敏原进行了研究：细菌提取物、细菌悬液以及来自溶血性链球菌和白色葡萄球菌的两者混合物。通过对22例对溶血性链球菌致敏的患者、23例对白色葡萄球菌致敏的患者以及10名健康人同时进行皮肤皮内试验，测定了不同浓度下所有过敏原的比活性。研究结果表明，细菌提取物主要引起速发型皮肤过敏反应，而细菌悬液则导致迟发型反应。混合过敏原可引起两种类型的皮肤反应，并且更具体地显示出对细菌的致敏作用。作者建议，用于皮内试验诊断的混合细菌过敏原的最佳浓度为10,000 PNU/ml和1亿个细菌/ml。文中讨论了三种细菌过敏原的优缺点。
