Pham V N, Roman B L, Weinstein B M
Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, Maryland 20892, USA.
Dev Dyn. 2001 Aug;221(4):470-4. doi: 10.1002/dvdy.1157.
The Tie1 and Tie2 receptor tyrosine kinases and the Tie2 ligands, the angiopoietins, play critical roles in vertebrate vascular embryogenesis, helping to mediate the interaction between endothelial cells and the pericytes or vascular smooth muscle cells that envelop and support them. We have obtained full-length cDNA sequences for zebrafish orthologs of angiopoietin-1 (ang1), angiopoietin-2 (ang2), and angiopoietin-like-3 (angptl3). Ang1 is expressed in head ventral mesenchyme, in the ventromedial region of somites, in mesenchyme surrounding trunk axial vessels, and in the hypochord, a transient embryonic structure of endodermal origin that has been implicated in dorsal aorta assembly in both zebrafish and Xenopus. Ang2 is expressed in head and anterior trunk ventral mesenchyme and the developing pronephric glomeruli. Angptl3 is expressed in the yolk syncytial layer.
Tie1和Tie2受体酪氨酸激酶以及Tie2配体血管生成素在脊椎动物血管胚胎发生过程中发挥着关键作用，有助于介导内皮细胞与包裹并支持它们的周细胞或血管平滑肌细胞之间的相互作用。我们已经获得了斑马鱼血管生成素-1（ang1）、血管生成素-2（ang2）和血管生成素样-3（angptl3）直系同源物的全长cDNA序列。Ang1在头部腹侧间充质、体节的腹内侧区域、围绕躯干轴向血管的间充质以及脊索中表达，脊索是一种内胚层起源的短暂胚胎结构，在斑马鱼和非洲爪蟾的背主动脉组装中都有涉及。Ang2在头部和躯干前部腹侧间充质以及发育中的前肾肾小球中表达。Angptl3在卵黄合胞体层中表达。
