Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Cambridge, MA; and.
Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA.
Blood Adv. 2018 Nov 13;2(21):3063-3069. doi: 10.1182/bloodadvances.2018021725.
Each stem cell resides in a highly specialized anatomic location known as the niche that protects and regulates stem cell function. The importance of the niche in hematopoiesis has long been appreciated in transplantation, but without methods to observe activity in vivo, the components and mechanisms of the hematopoietic niche have remained incompletely understood. Zebrafish have emerged over the past few decades as an answer to this. Use of zebrafish to study the hematopoietic niche has enabled discovery of novel cell-cell interactions, as well as chemical and genetic regulators of hematopoietic stem cells. Mastery of niche components may improve therapeutic efforts to direct differentiation of hematopoietic stem cells from pluripotent cells, sustain stem cells in culture, or improve stem cell transplant.
每个干细胞都存在于一个高度专业化的解剖位置，称为龛位，它可以保护和调节干细胞的功能。龛位在造血中的重要性在移植中很早就被认识到了，但由于缺乏在体内观察其活性的方法，造血龛位的组成成分和机制仍不完全清楚。在过去的几十年中，斑马鱼的出现为解决这个问题提供了答案。利用斑马鱼研究造血龛位，不仅使人们发现了新的细胞-细胞相互作用，还发现了造血干细胞的化学和遗传调控因子。对龛位成分的掌握可能会改善治疗效果，从而指导多能细胞向造血干细胞的分化，在培养中维持干细胞，或改善干细胞移植。
