Song Y, Ma H, Cai Z
Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100041.
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1999 May;15(3):175-7.
Lack of transplant material is a main problem in the repair and reconstruction of tissue or organ defects. To seek the succedaneum of transplants, we studied and used tissue-engineered growth hyaline cartilage.
The articular hyaline cartilage obtained from newborn calf within 6 hours of sacrifice was enzymatically dissolved. The chondrocytes were then seeded onto the medical collagen membrane of guided tissue regeneration in a 24 well plate in RPMI1640 medium. One week later, the chondrocyte medical collagen membrane of guided tissue regeneration complex was implanted subcutaneously into the back of nude mice. Eight weeks after implantation, the animals were sacrificed.
The present study seems to demonstrate that the cartilage-like tissue was strong enough to be transferred after being implanted for 8 weeks. The cartilage-like tissue was proved to be tissue-engineered hyaline cartilage by HE stain. The chondrocytes could secrete chondroitin sulfate as proved by Lev-Spicer stain.
Tissue engineering technique was used to make tissue-engineered cartilage in vitro. It will provide a new transplant material for the repair of tissue or organ defects.
移植材料的短缺是组织或器官缺损修复与重建中的主要问题。为寻找移植替代物，我们研究并应用了组织工程化生长透明软骨。
取出生6小时内宰杀的新生小牛的关节透明软骨，酶解后将软骨细胞接种于24孔板中含引导组织再生的医用胶原膜上，置于RPMI1640培养基中培养。1周后，将引导组织再生复合软骨细胞医用胶原膜皮下植入裸鼠背部。植入8周后处死动物。
本研究似乎表明，植入8周后的软骨样组织已足够坚韧，可以进行移植。经HE染色证实该软骨样组织为组织工程化透明软骨。经Lev-Spicer染色证实软骨细胞能分泌硫酸软骨素。
应用组织工程技术在体外构建组织工程化软骨，可为组织或器官缺损的修复提供一种新的移植材料。
