Zhang R G, Wang X W, Yuan J H, Xie H
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Acta Pharmacol Sin. 2000 Aug;21(8):742-6.
To investigate the effects of human telomerase RNA component antisense oligodeoxyribonucleotides on telomerase activity of human hepatoma cells and their effects on cell cycle distribution.
Modified telomeric repeat amplification protocol was used to detect telomerase activity. Cell cycle was analyzed by flow cytometer.
Telomerase activity was detected in all of four human hepatoma cell lines but absent in normal liver cells. Antisense oligomers to human telomerase RNA component (hTR) inhibited telomerase activity of BEL-7404 human hepatoma cells markedly in vitro. After in vitro treatment with antisense oligomers for 96 h, cell cycle of BEL-7404 human hepatoma cells was mainly arrested at G2/M phase.
Antisense oligomers to hTR inhibited telomerase activity of BEL-7404 human hepatoma cells in vitro and resulted in cell cycle arrest at G2/M phase.
研究人端粒酶RNA组分反义寡脱氧核苷酸对人肝癌细胞端粒酶活性的影响及其对细胞周期分布的作用。
采用改良的端粒重复序列扩增法检测端粒酶活性。用流式细胞仪分析细胞周期。
在4种人肝癌细胞系中均检测到端粒酶活性，而正常肝细胞中未检测到。人端粒酶RNA组分（hTR）反义寡聚体在体外能显著抑制BEL-7404人肝癌细胞的端粒酶活性。用反义寡聚体体外处理96小时后，BEL-7404人肝癌细胞的细胞周期主要阻滞在G2/M期。
hTR反义寡聚体在体外抑制了BEL-7404人肝癌细胞的端粒酶活性，并导致细胞周期阻滞在G/M期。
