Liu Y, Tay J H
Environmental Engineering Research Centre, School of Civil and Structural Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2001;36(6):1027-40. doi: 10.1081/ese-100104129.
Nitrite build-up in a nitrifying biofilm reactor for direct denitrification from the nitrite stage was investigated. At least three factors were found to influence the nitrite build-up: (i) the relative specific growth rates of Nitrosomonas to Nitrobacter, microNs/microNb in the biofilm; (ii) the relative initial ratio between Nitrosomonas and Nitrobacter on the support surface, (Mao)Ns/(Mao)Nb; (iii) the level of free ammonia, particularly at greater than 0.1 mg N/l that can be inhibitory to Nitrobacter. Results showed that without free ammonia inhibition, the first two factors determined the degree of nitrite build-up. The effect of (Mao)Ns and (Mao)Nb on nitrification kinetics were more significant than the specific growth rates of Nitrosomonas and Nitrobacter. By regulating effects of these three factors, extremely high concentrations of nitrite build-up could occur in a biofilm reactor. This offers a potential for achieving direct denitrification through a nitrate shunt.
研究了用于从亚硝酸盐阶段直接进行反硝化的硝化生物膜反应器中亚硝酸盐的积累情况。发现至少有三个因素会影响亚硝酸盐的积累：（i）生物膜中硝化单胞菌与硝化杆菌的相对比生长速率，即生物膜中的μNs/μNb；（ii）载体表面硝化单胞菌与硝化杆菌的相对初始比例，即（Mao）Ns/（Mao）Nb；（iii）游离氨的水平，特别是当游离氨浓度大于0.1 mg N/L时，可能会对硝化杆菌产生抑制作用。结果表明，在没有游离氨抑制的情况下，前两个因素决定了亚硝酸盐的积累程度。（Mao）Ns和（Mao）Nb对硝化动力学的影响比硝化单胞菌和硝化杆菌的比生长速率更为显著。通过调节这三个因素的作用，生物膜反应器中可能会出现极高浓度的亚硝酸盐积累。这为通过硝酸盐旁路实现直接反硝化提供了潜力。
