Chmelárová Z, Závadská I, Húska J, Tóth D
Institute of Microbiology, Slovak Academy of Sciences, 814 34 Bratislava, Slovakia.
Folia Microbiol (Praha). 2000;45(6):493-5. doi: 10.1007/BF02818716.
Starvation for carbon and energy sources accelerated the biotransformation of the anion-active surfactant dihexyl sulfosuccinate (DHS) by Comamonas terrigena cells. Chloramphenicol (Cm) added at different time intervals to non-starved cells inhibited the DHS transformation. The largest difference between cells treated and non-treated by Cm was observed for a 16-h-starvation period. Protein synthesis de novo during starvation enhanced the DHS biotransformation efficiency. A partial transformation of DHS in the presence of Cm indicated the constitutive character of enzymes involved in primary DHS biodegradation.
碳源和能源匮乏加速了陶氏丛毛单胞菌细胞对阴离子活性表面活性剂磺基琥珀酸二己酯（DHS）的生物转化。在不同时间间隔向未饥饿的细胞中添加氯霉素（Cm）会抑制DHS的转化。在16小时饥饿期观察到用Cm处理的细胞和未处理的细胞之间的最大差异。饥饿期间重新合成蛋白质提高了DHS的生物转化效率。在Cm存在下DHS的部分转化表明参与DHS初级生物降解的酶具有组成型特性。
