Lewan L, Petersen I, Yngner T
Hoppe Seylers Z Physiol Chem. 1975 Apr;356(4):425-9. doi: 10.1515/bchm2.1975.356.1.425.
Mouse kidney and liver were found to increase their levels of radioactivity above that of serum from 2 to 60 min after administration of [6-14C]orotic acid. In spleen, thymus and brain, the radioactivity level reached a maximum soon after the injection and then decreased, as did that in serum. Sixty minutes after the injection, 44% of the administered isotope dose was found in the kidneys, 22% in the liver and 0.75% in the spleen. The 14C activity in liver UTP increased rapidly and then remained constant for 60 min. The ratio between the activities in uridine phosphates and UDP-sugars was 3:4 from 10- 60 min after injection. In the liver and kidneys, the RNA 14C activities at 60 min after injection were 15% of the activity in their acid-soluble fractions. Intraperitoneal administration was found to be preferable to intravenous administration for studies on nucleotides and RNA in mouse liver, due to the delayed incorporation of the [14C]orotic acid activity into the nucleotide pool.
在给予[6-¹⁴C]乳清酸后2至60分钟，发现小鼠肾脏和肝脏中的放射性水平高于血清。在脾脏、胸腺和大脑中，注射后放射性水平很快达到最大值，然后下降，血清中的情况也是如此。注射60分钟后，发现给予的同位素剂量中有44%在肾脏中，22%在肝脏中，0.75%在脾脏中。肝脏UTP中的¹⁴C活性迅速增加，然后在60分钟内保持恒定。注射后10至60分钟，尿苷磷酸和UDP-糖中的活性之比为3:4。在肝脏和肾脏中，注射60分钟后RNA的¹⁴C活性是其酸溶性部分活性的15%。由于[¹⁴C]乳清酸活性掺入核苷酸池的过程延迟，发现腹腔注射比静脉注射更适合用于小鼠肝脏中核苷酸和RNA的研究。
