部分肝切除术后大鼠和小鼠肝脏中[14C]乳清酸摄取与UTP和RNA标记之间的相互关系。

Interrelations between uptake of [14C]orotic acid and labeling of UTP and RNA in rat and mouse liver after partial hepatectomy.

作者信息

Yngner T, Carlberg E, Lewan L, Engelbrecht C

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Aug;360(8):1069-74. doi: 10.1515/bchm2.1979.360.2.1069.

DOI:10.1515/bchm2.1979.360.2.1069

Abstract

The uptake and utilization of [6-14C]orotic acid for UTP and RNA synthesis were studied in rat and mouse liver at 24 h after partial hepatectomy. Rat liver concentrated radioactivity relative to blood several-fold better than did mouse liver after both sham-operation and partial hepatectomy. The results showed that in mouse liver, contrary to rat liver, the orotic acid uptake was not increased after the partial hepatectomy. In rat liver, the precursor uptake and the labeling of UTP increased by about 75% whereas the specific radioactivity of RNA increased 2 to 3-fold after the operation, thus indicating an increased RNA synthesis. Mouse liver showed no increased [14C]orotic acid uptake or labeling of UTP or RNA at 24 h after partial hepatectomy.

摘要

在部分肝切除术后24小时，研究了大鼠和小鼠肝脏对[6-14C]乳清酸用于合成UTP和RNA的摄取及利用情况。假手术和部分肝切除术后，大鼠肝脏相对于血液对放射性的浓缩能力比小鼠肝脏强几倍。结果显示，与大鼠肝脏相反，在小鼠肝脏中，部分肝切除术后乳清酸摄取并未增加。在大鼠肝脏中，手术前后前体摄取及UTP的标记增加了约75%，而RNA的比放射性增加了2至3倍，这表明RNA合成增加。部分肝切除术后24小时，小鼠肝脏中[14C]乳清酸摄取、UTP或RNA的标记均未增加。