Sharma Anita, Pandey Arvind, Sharma Shashi, Chatterjee Indranil, Mehrotra Ravi, Sehgal Ashok, Sharma Joginder K
Division of Molecular Diagnostics, Institute of Cytology and Preventive Oncology (ICMR), I-7, sector 39, NOIDA-201301, India.
Centre for Biotechnology, University of Allahabad, Allahabad- 211001, India ; Division of Molecular Oncology, Institute of Cytology and Preventive Oncology (ICMR), I-7, NOIDA, India.
Meta Gene. 2014 Jan 20;2:134-42. doi: 10.1016/j.mgene.2013.12.003. eCollection 2014 Dec.
Glutathione S-transferases (GSTs) belong to a super family of phase II detoxification enzymes, which play an important role in protecting cells from damage caused by endogenous and exogenous compounds by conjugating reactive intermediates with glutathione to produce less reactive water-soluble compounds. In the present study, we determined the frequencies of two polymorphisms in exon 5 and exon 6 of GSTP1 gene in 500 normal individuals from Delhi. GSTP1 polymorphism was analysed by PCR-RFLP using amplification refractory mutation system (ARMS) assay. Two polymorphic sites in GSTP1 (Ile105 → Val105; Ala114 → Val114) have been analysed simultaneously, which results in four alleles: GSTP1A (wild-type Ile105; Ala114), GSTP1B (Val105; Ala114), GSTP1C (Val105; Val114) and GSTP1D (Ile105; Val114). The GSTP1 allele frequency in Delhi population was 0.663, 0.248, 0.069, and 0.020 for GSTP1A, GSTP1B, GSTP1C, and GSTP1D respectively. The frequency of Ile105 and Val105 allele was 0.683 and 0.317 respectively and it was calculated for the purpose of comparison with published data where all the four alleles were not analysed. GSTP1 alleles from Delhi population were compared with reported frequencies from all over India, and from other ethnic groups worldwide. This study would provide a basic database for future genetic studies.
谷胱甘肽S-转移酶（GSTs）属于II期解毒酶的一个超家族，通过将反应性中间体与谷胱甘肽结合以产生反应性较低的水溶性化合物，在保护细胞免受内源性和外源性化合物引起的损伤方面发挥重要作用。在本研究中，我们测定了来自德里的500名正常个体中GSTP1基因第5外显子和第6外显子中两种多态性的频率。使用扩增不应性突变系统（ARMS）分析法通过PCR-RFLP分析GSTP1多态性。同时分析了GSTP1中的两个多态性位点（Ile105→Val105；Ala114→Val114），这产生了四个等位基因：GSTP1A（野生型Ile105；Ala114）、GSTP1B（Val105；Ala114）、GSTP1C（Val105；Val114）和GSTP1D（Ile105；Val114）。德里人群中GSTP1等位基因频率对于GSTP1A、GSTP1B、GSTP1C和GSTP1D分别为0.663、0.248、0.069和0.020。Ile105和Val105等位基因频率分别为0.683和0.317，计算该频率是为了与未分析所有四个等位基因的已发表数据进行比较。将德里人群的GSTP1等位基因与全印度以及全球其他种族群体报告的频率进行了比较。本研究将为未来的基因研究提供一个基础数据库。
