Haughey S A, Baxter G A, Elliott C T, Persson B, Jonson C, Bjurling P
Department of Agriculture and Rural Development, Veterinary Sciences Division, Stormont, Belfast, UK.
J AOAC Int. 2001 Jul-Aug;84(4):1025-30.
Clenbuterol (CBL) is an orally active beta2-adrenoceptor agonist which has been used in veterinary medicine as a broncodilator and an agent of uterine relaxation. It has however become better known as a drug used illegally to promote growth in farm animals. A rapid and sensitive biosensor assay was developed to detect CBL residues in bovine urine. The method involved a simple extraction procedure using tert-butyl methyl ether followed by analysis on the biosensor with results obtained against a buffer calibration curve. The assay allowed up to 88 samples to be analyzed per working day, with each cycle on the biosensor taking approximately 7 min to complete. The limit of detection (LOD) was determined as 0.27 ng/mL using 20 EU reference blank urine samples. The intra-assay Sr ranged from 4.7-7.6% for 3 control samples while the interassay Sr ranged from 9.2-12.7%. The recovery was found to be approximately 95%. A series of incurred urine samples were assayed and the results compared by Enzyme immunoassay (EIA), radio-immunoassay (RIA), and gas chromatography/mass spectrometry (GC/MS) analysis. Urine samples taken from local abattoirs were also analyzed by the biosensor method and by EIA analysis. The antibody used in the biosensor test exhibited high cross reactivity with at least 7 other beta-agonists allowing detection of these compounds at less than 1 ng/mL in bovine urine.
克伦特罗（CBL）是一种口服活性β2肾上腺素能受体激动剂，曾在兽医学中用作支气管扩张剂和子宫松弛剂。然而，它因被非法用于促进农场动物生长而更为人所知。开发了一种快速灵敏的生物传感器检测方法来检测牛尿液中的克伦特罗残留。该方法包括使用甲基叔丁基醚进行简单的提取步骤，然后在生物传感器上进行分析，并根据缓冲液校准曲线获得结果。该检测方法每个工作日最多可分析88个样品，生物传感器上的每个循环大约需要7分钟完成。使用20份欧盟参考空白尿液样品，检测限（LOD）确定为0.27 ng/mL。3个对照样品的批内相对标准偏差（Sr）范围为4.7 - 7.6%，批间Sr范围为9.2 - 12.7%。回收率约为95%。对一系列实际尿液样品进行了检测，并通过酶免疫分析（EIA）、放射免疫分析（RIA）和气相色谱/质谱联用（GC/MS）分析对结果进行了比较。还通过生物传感器方法和EIA分析对从当地屠宰场采集的尿液样品进行了分析。生物传感器检测中使用的抗体与至少7种其他β激动剂表现出高交叉反应性，能够在牛尿液中以低于1 ng/mL的浓度检测到这些化合物。
