Hahnau S, Jülicher B
EU Reference Laboratory for Residues of Veterinary Drugs, Federal Institute for Health Protection of Consumers and Veterinary Medicine, Berlin, Germany.
Food Addit Contam. 1996 Apr;13(3):259-74. doi: 10.1080/02652039609374408.
This article presents the results of a comparative study of nine commercially available ELISA test kits for clenbuterol and other beta 2-agonists currently being used in veterinary drug residue control of live and slaughtered animals in the European Union (EU). By determining measuring ranges, B/B0-50% values, inter- and intra-assay variations and cross-reactions as well as limits of detection (LOD) and recoveries for clenbuterol in bovine urine it could be demonstrated that a number of test kits showed considerable quality defects, reducing their applicability to residue control. LODs for urine assayed without a previous clean-up (as recommended by all kit manufacturers) varied between 1.2 and 11.1 ng clenbuterol per ml urine and did thus not meet the requirement of 1 ng/ml of the official residue control plans of the EU Member States.
本文介绍了对九种市售的用于检测克伦特罗和其他β-2激动剂的酶联免疫吸附测定（ELISA）试剂盒的比较研究结果，这些试剂盒目前用于欧盟（EU）对活体和屠宰动物兽药残留的控制。通过测定牛尿中克伦特罗的测量范围、B/B0-50%值、批内和批间变异以及交叉反应，以及检测限（LOD）和回收率，可以证明一些试剂盒存在相当大的质量缺陷，降低了它们在残留控制中的适用性。未经预先净化（所有试剂盒制造商均推荐）的尿液检测的LOD在每毫升尿液1.2至11.1纳克克伦特罗之间，因此不符合欧盟成员国官方残留控制计划中1纳克/毫升的要求。
