Mossoba M M, Adam M, Lee T, Bastyr J
US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Scientific Analysis and Support, Washington, DC 20204, USA.
J AOAC Int. 2001 Jul-Aug;84(4):1144-50.
Interest in trans fat labeling has prompted efforts to develop new, more efficient methods for rapidly and accurately determining trans fat content of foods. A novel and rapid (5 min) attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopic procedure was recently developed and applied to food products. This procedure was voted official method AOCS Cd 14d-99 by the American Oil Chemists' Society in 1999 after testing in a 12 laboratory international collaborative study. The results of the study are described in this paper. Analytical ATR-FTIR results exhibited high accuracy in the range 5-40% trans; results tended to have <2% high bias relative to the gravimetrically determined values. The precision of this internal reflection method was found to be superior to the precision of transmission infrared official methods. It is recommended that the applicability of the ATR-FTIR method be limited to trans levels of >5% (as percent of total fat).
对反式脂肪标签的关注促使人们努力开发新的、更高效的方法，以快速准确地测定食品中的反式脂肪含量。最近开发了一种新颖且快速（5分钟）的衰减全反射傅里叶变换红外（ATR-FTIR）光谱法，并将其应用于食品。该方法在一项由12个实验室参与的国际合作研究中经过测试后，于1999年被美国油脂化学家协会投票选为官方方法AOCS Cd 14d-99。本文描述了该研究的结果。分析性ATR-FTIR结果在反式脂肪含量为5%-40%的范围内显示出高精度；相对于重量法测定值，结果往往有<2%的正偏差。发现这种内反射方法的精密度优于透射红外官方方法。建议ATR-FTIR方法的适用性仅限于反式脂肪含量>5%（占总脂肪的百分比）的水平。
