Raisi F, Belgrader P, Borkholder D A, Herr A E, Kintz G J, Pourhamadi F, Taylor M T, Northrup M A
Cepheid, Sunnyvale, CA 98089, USA.
Electrophoresis. 2001 Jul;22(11):2291-5. doi: 10.1002/1522-2683(20017)22:11<2291::AID-ELPS2291>3.0.CO;2-A.
A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real-time distribution of fluorescently labeled peptides subjected to gel-free mIEF. During the run, the effective length of the 6-cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements.
