Shihabi Z K, Hinsdale M E, Cheng C P
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Electrophoresis. 2001 Jul;22(11):2351-3. doi: 10.1002/1522-2683(20017)22:11<2351::AID-ELPS2351>3.0.CO;2-C.
Glutathione is a small peptide, which participates in cellular oxidation-reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis based on stacking. After tissue homogenization or hemolysis of the red blood cells, the samples were deproteinized with acetonitrile and injected filling about 13% of the capillary volume. The electrophoresis was performed at 10 kV using a separation buffer of 250 mM borate, 50 mM Tris, pH 8.0. Sample stacking increased the sensitivity of detection by 10-20-fold.
谷胱甘肽是一种小肽，参与细胞氧化还原和解毒过程。它以不同浓度存在于大多数生物组织中。基于堆积的毛细管电泳法测定了红细胞和心肌组织中该肽的氧化型和还原型。组织匀浆或红细胞溶血后，样品用乙腈脱蛋白，进样量约为毛细管体积的13%。电泳在10 kV下进行，使用250 mM硼酸盐、50 mM Tris、pH 8.0的分离缓冲液。样品堆积使检测灵敏度提高了10 - 20倍。
