Yokotsuka K, Kikuchi A, Aihara T, Kushida T
J Biochem. 1975 Apr;77(4):817-21. doi: 10.1093/oxfordjournals.jbchem.a130788.
Whole histone from calf thymus was fractionated by partition chromatography on the basis of distribution between an aqueous phase immobilized on Sephadex G-25 beads and mobile organic phases containing various concentrations of trichloroacetic acid. The chromatography was carried out by stepwise elution with five upper phases from water and butanol-2 solvent systems containing 4 M urea and 0.1-1.5% trichloroacetic acid, and finally water. Of the six peaks obtained, two (peaks 1 and 2) contained arginine-rich histones. Although these peaks were still heterogeneous electrophoretically, the band corresponding to F2al was observed only in the electrophoretic pattern of peak 1 and the main fraction in peak 2 was F3. A histone fraction having nearly equimolar amounts of arginine and lysine was obtained from peak 3. Its amino acid composition was similar to that of F2a2. Slightly lysine-rich histone obtained from peak 4 showed an amino acid composition typical of F2b. Peak 5 contained a histone fraction with a ratio of lysine/arginine of 6.14, showing a single band on gel-electrophoresis. Very lysine-rich histone (F1) was obtained from peak 6, and the electrophoretic pattern of this fraction showed a single band.
基于固定在葡聚糖凝胶G - 25珠上的水相和含有不同浓度三氯乙酸的流动有机相之间的分配，通过分配色谱法对来自小牛胸腺的全组蛋白进行分级分离。色谱分离是通过用来自含有4M尿素和0.1 - 1.5%三氯乙酸的水和2 - 丁醇溶剂系统的五个上层相逐步洗脱，最后用水洗脱来进行的。在得到的六个峰中，两个峰（峰1和峰2）含有富含精氨酸的组蛋白。尽管这些峰在电泳上仍然是异质的，但仅在峰1的电泳图谱中观察到对应于F2al的条带，峰2中的主要组分是F3。从峰3获得了一种精氨酸和赖氨酸含量几乎等摩尔的组蛋白组分。其氨基酸组成与F2a2相似。从峰4获得的略富含赖氨酸的组蛋白显示出F2b典型的氨基酸组成。峰5含有一种赖氨酸/精氨酸比率为6.14的组蛋白组分，在凝胶电泳上显示为单一条带。从峰6获得了非常富含赖氨酸的组蛋白（F1），该组分的电泳图谱显示为单一条带。
