Pfeifhofer A O, Belton J C
J Cell Sci. 1975 Jul;18(2):287-99. doi: 10.1242/jcs.18.2.287.
The structure of chloroplasts isolated from Dunaliella salina has been studied with respect to changing concentrations of sodium chloride in the culture medium. Freeze-fracture replicas and thin sections of intact chloroplasts do not exhibit any noticeable changes in structure at concentrations ranging between 3.5 and 25% NaCl. Chloroplasts isolated from algal cells that have been acclimatized to the higher salt concentration show a change in the thylakoid membranes. The thylakoid membranes appear compressed over a major portion of the membrane surface, with only the end of the thylakoid membranes unappressed. The number of particles per unit area on the B face is also altered by the salt concentration. The chloroplasts acclimatized to 25% NaCl have about 3 times the number of particles per unit area on a B face of end-membranes as on a comparable face of thylakoid membranes acclimatized to low (3.5% NaCl) salt concentration. These morphological changes can be reversed if the chloroplasts acclimatized to high or low salt concentrations are returned to a medium of different salt concentration prior to freeze-fracturing.
针对培养基中氯化钠浓度的变化，对从盐生杜氏藻中分离出的叶绿体结构进行了研究。在3.5%至25%氯化钠浓度范围内，完整叶绿体的冷冻蚀刻复制品和薄片在结构上未表现出任何明显变化。从适应较高盐浓度的藻类细胞中分离出的叶绿体，其类囊体膜会发生变化。类囊体膜在膜表面的大部分区域似乎受到挤压，只有类囊体膜的末端未受挤压。B面上单位面积的颗粒数量也会因盐浓度而改变。适应25%氯化钠的叶绿体，其内膜B面上单位面积的颗粒数量约为适应低盐浓度（3.5%氯化钠）的类囊体膜相应表面上颗粒数量的3倍。如果在冷冻蚀刻之前，将适应高盐或低盐浓度的叶绿体放回不同盐浓度的培养基中，这些形态变化是可以逆转的。
